正常威年小鼠脊髓内部结构的三维重建中国数字医学.PDFVIP

正常威年小鼠脊髓内部结构的三维重建 吴辉群①耿兴云①蒋葵①吕广明⑦韩笑②季这峰②董建成+ 摘要 小鼠脊髓内部结构的三维重建对于“人工脊髓”生物材料的制备具有重要意义，因此．我们利用小鼠脊髓参照图 谱和三维重建工具软件对小鼠脊髓内部结构进行三维重建。方法是获得小鼠脊髓参照图谱．并利用小鼠脊髓Nissl和LFB染 的板层和主要传导束进行分割和三维重建。结果重建出小鼠脊髓外形、脊髓灰质、板层、皮质脊髓束、薄束、楔束等结 构．重建的结构具有立体感，并能在PC机中任意角度观察。 关键词三维重建连续切片小鼠脊髓3D—DOCTOR Doi：10．3969／j．issn．1673—7571．2009．09．006 jn ThreeDimensionaIReconstructionofInnerStructuresCordofNormaIAdultMouse／WU Spinal Hui—qun．GENGXing— al／／China Medicine．一2009to26 Kui，et 4(9)：24 yun，JIANG Digital in AbstractThe3Dreconstructionofinnerstructurescordofmousejs tothe ofbiomaterials spinal significantpreparation for”artificiaI makethree ofinnerstructuresin cordof cord。．So，we dimensionaI(3D)reconstruction spinal spinal 3D mousewithmousecordreferenceatlasand reconstructionsoftware．Itisthemethodsthatthemouse spinal spinal cordreferenceatlaswasobtainedand theNisslandLFB software provedby stainingcryosections．ThePhotoshop on wasusedtomake andbatchtreatmentobtained the3D-DOCTORsoftwarewasusedto registration atlas，and make and3Dreconstructionoflaminaeand matter，laminae， segmentation majortracts．Finally，thespinalcord，grey were reconstructedandcanbeobserved inPC． corticospinaltracts．gracile，cuneatesuccessfully arbitrarily 3D reconstruction。Serialsections．Mousecord．3D—DOCTOR Keywords Spinal Fund Fu