观察洋葱表皮实验中碘液的配制（The preparation of iodine solution in onion skin experiment was observed）.docVIP

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观察洋葱表皮实验中碘液的配制（The preparation of iodine solution in onion skin experiment was observed）.doc

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观察洋葱表皮实验中碘液的配制（The preparation of iodine solution in onion skin experiment was observed）

观察洋葱表皮实验中碘液的配制（The preparation of iodine solution in onion skin experiment was observed） Not only saves time, but also avoids the disadvantages. 1., the preparation of salt dilute iodine solution (1) dissolve 0.2 g of iodine with 95% concentrated alcohol 70 g; (2) dissolve 0.9 g of salt with 30 g distilled water; (3) matters needing attention: Dissolve iodine, dont use water as solvent, because of the low solubility of iodine in water, and two because water soluble iodine solution is easy to deteriorate. The concentration of alcohol in the diluted iodine solution of the salt is better than about 60%, and it is not volatile and can be preserved for a long time, and the remaining iodine solution can also be used for the examination of the digestion of the starch with the saliva amylase, without wasting the medicine. Alcohol insoluble in salt. Methods 2. gentian violet liquid preparation (1) 100 g distilled water containing 2 beakers, 0.9 g and 0.1 g were dissolved violet. Then mix the two fluids. (2) in a dissolved salt and gentian violet in the beaker is not good, so difficult to dissolve gentian violet. 3. of two dyes compared to gentian violet solution as well, it was more obvious than iodine staining nuclei. 4. directions for the improvement of the experiment Drip saline and staining should be carried out in two steps, and no material has been described theoretically. I think the cells are in normal saline to keep isotonic, and the progressive staining is not isotonic, but the cells are in non isotonic condition after dyeing. I made two steps together to make the cells stay under the isotonic condition for a long time, which is more in line with the textbook. And the author made a comparative observation, and changed the two step to another step. The effect of dyeing was equally good. Iodine liquid preparation: 0.01mol/l iodine liquid - called 1.3g iodine, placed in 50mol beaker, adding 2.5G potassium iodide and 25 ml distilled water, stirring constantly, so that