胰岛素样因子羟基磷灰石加自体红骨髓治疗骨缺损研究.DOCVIP

IGF-I活化组织工程骨修复骨缺损的实验研究 邓 江(，黄文良（） 目的 探讨胰岛素样生长因子-I（IGF-I）羟基磷灰石（HA）及自体红骨髓（ABM）的效果。方法 随机分组空白 HA/ABM组、IGF-IHA组、IGF-IHA/ABM组组织工程骨分别植入骨缺损处。术后于分别对动物的成骨情况按Lane-Sandh组织学评分标准进行评分。结果 结果显示IGF-IHA/ABM组与其他各组比较P 0.05)。结论 IGF-IHA为支架材料组织工程骨的成骨诱导作用明显增强。 【关键词】胰岛素样生长因子-I；组织工程骨；骨缺损；成骨诱导 The experimental study of repairing bone defect by IGF-I activated tissue engineered bone complex /Deng Jiang, Huang Wenliang, HanXiaosong, Xu Lin,WangShiqiang. Department of Orthopaedics, The hospital of Zunyi, Zunyi, 563002, P.R.China. Corresponding author:Deng Jiang. 【Abstract】　Objective o investigate the efficacy of insulin-like growth factor-I(IGF-I) osteoinduction active on hydroxyapatite (HA)/ autogenous red bone marrow(ABM). Methods The animal model of bone defect was made on the bilateral radius of 36 rabbits, which were randomly divided into four groups. Defects were repaired with different transplant materials:with no implant in the blank control group;with implant HA/ABM group; with implant IGF-I activated HA group; with implant IGF-I activated HA/ABM group. The examination of the level of serum alkaline phosphatase, X-ray exam, histology observation and scanning electronic microscope exam were performed at each times. Results each times and groups result analysis: group IGF-I activated HA/ABM complex stimulated more bone formation than others. The difference of reconstructive ability has significant statistical significance compared with others(P 0.05) by the scores of Lane-Sandhu X-ray and histology. Conclusion the IGF-I could significantly Increased osteoinduction active on tissue engineering bone produced by HA as scaffold material. 【Key words】insulinlike growth factor-I; tissue engineered bone; bone defect; osteoinduction 胰岛素样生长因子-I (IGF-I)是一种具有多种生物学活性的骨生长调节因子，能刺激成骨细胞和成骨前体细胞的增殖、分化和基质合成，并通过介导作用调节破骨细胞的分化形成和功能活性，在骨改建耦联中发挥重要作用[1]。笔者研究旨在应用IGF-I活化CHA/ABM，以提高组织工程骨的成骨诱导活性，促进成骨细胞形成，为骨组织工程提供良好的种植体。 材料与方法 1 材料准备 1.1 HA准备：HA（上海睿星基因技术有限公司）多孔生物材料，孔隙率为60%~80%，孔径为100~300μm，大小为2.0cm×0.5cm×0.5