双波长反相高效液相色谱法同时测定葛根姜黄.PDF

6 8 Vol. 6 No. 8 2015 8 Journal of Food Safety and Quality Aug. , 2015 * 苏昭仑 , 黄康惠, 张喜金, 陈彩云, 陈宏壁, 林贤邦 (, 519040) 摘 要: 目的 方法 50%, SUPELCO-C18 , -, , 0~25 min 230 nm, 25.01~39 min 430 nm 结果 0.01~0.1 mg/mL; 96.3~103.3%, RSD2.0% 结论 , , , 关键词: ; ; ; ; Determination of puerarin, paeoniflorin and curcumin in radix puerariae turmeric collagen tablets by reversed-phase high performance liquid chromatography with dual-wavelength * SU Zhao-Lun , HUANG Kang-Hui, ZHANG Xi-Jin, CHEN Cai-Yun, CHEN Hong-Bi, LIN Xian-Bang (By-Health Co. , Ltd., Zhuhai 519040, China) ABSTRACT: Objective To establish a method for the determination of puerarin, paeoniflorin and curcumin in radix puerariae turmeric collagen tablets by reversed-phase high performance liquid chromatography. Methods Samples were ultrasonically extracted with 50% ethanol. The extracts were separated by SUPELCO-C18 column with gradient elution and methanol-water as the mobile phase, then samples were detected between 0~25 min at a wavelength of 230 nm and 25.01~39 min at a wavelength of 430 nm. Results Puerarin, paeoniflorin and curcumin could be completely separated by the conditions. The linear range was 0.01~0.1 mg