神经酰胺转运蛋白在非囊泡转运中的作用机制.pdfVIP

ISSN 1007-7626 20094 CN 11-3870PQ Chinese Journal of Biochemistry and olecular Biology 25(4):316~ 320 #综述# * 许佳丽, 郭 军 (南京医科大学基础医学实验教学中心, 南京 210029) 神经酰胺转运蛋白(ceramide transfer protein, CERT) 是介导神经酰胺( ceramide)非囊泡转运的 载体. 它 括3 个功能区域: PHFFAT 和START.PH 和FFAT 分别发挥高尔基体和内质网的靶向作 用,羧基端的START 主要用于与神经酰胺结合. CERT 的转运受多种因素的调节, 依赖于PKD 和 PP2CE诱导的丝氨酸重复区域(SR) 的磷酸化和去磷酸化, 氧化应激刺激的CERT 三聚体形成,以及 PI4KÓB 催化的高尔基体接头PI4P的生成等.CERT 功能障碍会导致细胞易受氧化应激的损害.本 文拟从CERT 的结构作用及其调节机制3 方面进行综述,揭示CERT 的研究进展. 神经酰胺; 神经酰胺转运蛋白; 非囊泡运输; 调节机制 Q71 Molecular Mechanism of CERT in Non-vesicular Transfer * XU Jia-Li, GUO Jun (Laboratory Center f or Bas ic Medical Sciences ,Nanj ing Medical University ,N anj ing 210029, China ) Abstract Ceramide transfer protein ( CERT) has been identified as a key factor in non-vesicular transfer of ceramide from ER to Golgi apparatus. CERT contains several functional domains including a PH (pleckstrin homology) domain, aFFAT (two phenylalanines in an acidictract) motif and aSTART domain. Amongthese regions, the PH and FFAT are responsible forthe targeting theGolgi apparatus and ER, respectively. The C- terminalSTART domain is capable to bind ceramide. The transportation of ceramide is regulated via several mechanisms, such as the phosphorylation and dephosphorylation of serine repeat domain, in which protein kinaseD andPP2CE are involved. Oxidative stress is known to induce the rapid formation of a stable CERT homotrimer complex. PI4P, which istheproduction of phosphorylatedPIby PI4