分子遗传学-植物基因组学.pdfVIP

中国农业大学国家玉米改良中心 National Maize Improvement Center of China, CAU 植物基因组学Ⅱ Plant Genomics 杨小红 yxiaohong@cau.edu.cn 绪论 Introduction of Molecular Genetics What is Molecular Genetics? History of Molecular Genetics The Molecular Basis of Genetics What is gene? How gene expresses? DNA is the genetic material DNA Components DNA Structure DNA Replication Transcription Translation Gene Structure Plant Genomics What is genomics? The history of genomics How to study the genomics? Molecular markers Libraries Sequencing Characterization of genome How to study genomics? Mapping Sequencing Assemble 作图 测序 组装 Genetic Physical Map-based Random Framework Gap map map sequencing sequencing mapping mapping 遗传图 物理图 图谱测序 随机测序 骨架图 空隙填图 Coordinating Correcting Draft and completed 整合 校正 sequence 草图与精图 Physical map (物理图谱) is a map that shows the real positions of various genes or markers or DNA sequences on a genome (bp, kb, Mb). B73 reference sequence Protocol for physical mapping Chromosomes BAC Fingerprint Contig Integrated map Libraries DNA library cDNA library Full-length cDNA EST Genomic library Vector type Insert