生物质炭对水稻土中脱氢酶活性与铁还原过程的影响.docVIP

生物质炭对水稻土中脱氢酶活性和铁还原过程的影响 唐珺瑶，贾蓉，曲东(，李才，耿茂梧 摘要：为探究添加生物质炭对淹水稻田体系脱氢酶活性及土水界面铁还原过程的影响，选择2种不同地区水稻土，采用土壤泥浆厌氧培养试验方法，分析了添加不同粒径生物质炭后泥浆培养体系中脱氢酶活性、pH以及Fe(Ⅱ)浓度的变化。结果表明：生物质炭能够提高水稻土厌氧培养体系的脱氢酶活性、促进微生物铁还原进程。脱氢酶活性和铁还原能力随着生物质炭粒径的减小而增大。未添加生物质炭的处理中，两种水稻土脱氢酶活性最大为3.13、2.60 μg/(ml·g·min)，Fe(Ⅱ)累积量最高为8.07、7.44 mg/g；通过添加生物质炭，培养体系脱氢酶活性最大达4.35、4.18 μg/(ml·g·min)、Fe(Ⅱ)累积量最高为9.01、8.18 mg/g。典范对应分析显示水稻土初始pH与最大铁还原潜势及达到最大铁还原速率对应的时间极显著相关，表明生物质炭对铁还原过程的影响因土壤性质不同而存在差异；脱氢酶活性（DHA）、Fe(Ⅱ)累积量之间呈现极显著的相关关系，两者在培养过程中相互促进。 关键词：生物质炭；水稻土；脱氢酶；微生物铁还原 中图分类号：S153.3 Effects of Biochar on Dehydrogenase Activity and Iron Reduction in Paddy Soils TANG Junyao，JIA Rong， QU Dong*， LI Cai，GENG Maowu (College of Natural Resources and Environment，Northwest AF University，Yangling, Shaanxi 712100，China) Abstract：To investigate the effect of biochar on dehydrogenase activity and iron reduction in paddy soil，two?kinds of paddy soil in different areas were anaerobically incubated under the addition of biochar with different partical sizes. Changes of dehydrogenase activity，Fe(Ⅱ) concentration and pH were determined during incubation. Results showed that biochar promoted dehydrogenase activity and microbial Fe(Ⅲ) reduction. Dehydrogenase activity and iron reduction potential in paddy soils increased with the decrease of the biochar practical sizes. Dehydrogenase activity was 3.13 and 2.60 μg/(ml·g·min)，and Fe(Ⅱ) concentration was 8.07 and 7.44 mg/g without adding biochar in two paddy soils，respectively. With adding biochar，dehydrogenase activity arrived at 4.35 and 4.18 μg/(ml·g·min)，and Fe(Ⅱ) concentration was 9.01 and 8.18 mg/g. CCA showed a significant correlation between initial pH of paddy soils and iron reduction potential，which indicated that the effect of biochar on iron reduction was different due to the disparity of soil property. Dehydrogenase activity and Fe(Ⅱ) concentration showed a significant correlation，and both of them promoted each other during incubation. Key words：biochar；paddy soil；dehydrogenase；microbial iron reduc