细胞的运输（Cell transport）.docVIP

细胞的运输（Cell transport） The condition of adherent cells is not clear, but the suspension cells are OK and can be easily carried by humans as long as they are above zero. 1, culture bottles filled with culture medium 2 tighten the cap and seal the film 3, close your body and minimize vibrations 4, 24 hours, revert to the original state of culture The cell bottle should be filled with culture medium. Dont leave air. Otherwise, the movement of bubbles in the bottle will have a negative effect on the cells. As long as the cell seal and ensure sterile, transit across the vertical line. But I think its safer to put it upright. (without a reason) After the best cell to the laboratory temperature box for several hours (horizontally as well, so that the maximum number of adherent cells), to the buffer time. After you return the temperature, you can suck out the excess medium and continue to develop normally. Are culture medium filled with fetal bovine serum or calf serum? Yes, of course. Cells can grow normally in a full medium. When you return to the lab, remove the culture medium and dont throw it away. It can be used to culture the cell. Because the cells that are bought back are fragile, if they change the culture medium immediately (that is, the same medium, and there are differences between the two laboratories), it may affect cell morphology. So first use their medium to grow cells, and so on, to a certain size, and then use your own culture medium. Can cultivate time to be passaged in cells, fresh medium, adding a certain amount of sealed bottle, cover with foil, which can transport at room temperature within a few days, not what problem. Fill the medium to the neck of the bottle and seal the opening with a sealing film. One thing is more important. The cells are afraid of heat. Its so hot now, youd better take an air-conditioned car on the road, and keep the cells in your pocket. Thats how I came back from Beijing last month with a bottle of cells back to Hefei, and t