慢病毒介导RNAi沉默PIK3CA基因对卵巢上皮性癌细胞株SKOV3增殖和凋亡地影响.pdfVIP

中文摘要 想载体。 2 水平被显著、持久沉默。 3 显的调节作用。 细胞G2／M期细胞数减少，S期细胞数比例增加，可能是使 细胞周期阻滞于S期。 关键词： Real．time PCR；Western—Blot 英文摘要 Effectsof RNAi lentivirus--mediated inhibiting PIK3CAon of and ovarian apoptosisproliferation celllineSKOV3 cancer epithelial ABSTRACT ective：AlentiviralvectorofRNAi human obj targeting PIK3 CA ll0a)wasconstructedandthelentiviralvector gene(P wastransfected intohumanovariancancercelllineSKOV3． The ofPIK3wasdetected effect CA Real—time inhibitory by PCR andthe of ofSKOV3cells changesproliferation contransfectedwithlentivialvectorwereexaminedMTT． by FCMwas to the of and employed investigatechangesapoptosis cell ofSKOV3cells．Theaimofthe wasto cycle presentstudy the oflentivirus-mediatedPIK3CA exploreprospects silencing in forovariancancerinorderto applicationgenetherapy anewtreatmentforovariancancer．。 provide Methods：1TheRNAi PIK3CA sequencestargeting gene were as