甜菜与BNYVV互作过程中PR基因的诱导表达.docVIP

基金项目：国家自然科学基金资助项目。 作者简介：(1980-)，硕士研究生研究方向通讯作者：，，(1962-)，教授，博士生导师，。Tel：0471-4301709，E-mail：syzh36@。Inducible Expression of PR Gene in the Process of Interaction Between Sugar beet and BNYVV LIli1 ，ZHANG Shaoying1( 1. Sugarbeet Physiological Institute, Agricultural University of Inner- mongolia, Huhhot 010018, China) Abstract: Four sugar beet varieties including two resistant varieties and two susceptible ones were used in this experiment，the relationship between activities and the induced gene expression in different time of chitinase and β-1,3-glucanase in sugar beet with different disease resistance which inoculated beet necrotic yellow vein virus(BNYVV) were studied . The results showed: the activities of chitinase andβ-1,3-glucanase were enhanced to some extent，chitinase andβ-1,3-glucanase activities of the resistant varieties were higher than the susceptible ones ，and the highest chitinase andβ-1,3-glucanase activities of the resistant varieties were achieved earlier than the susceptible ones；the expression of chitinase、β-1, 3-glucanase genes of the resistant varieties were earlier than the susceptible ones, furthermore, the expression quantity of the resistant varieties were more than the susceptible ones and duration longer than the susceptible ones. Beet necrotic yellow vein virus (BNYVV) can activated defense response system by induced defense response gene expression in sugar beet to some extent, the induced expression of PR genes were enhanced to the resistance to Rhizomania. Key words: Sugar beet，Rhizomania，chitinase，β-1, 3-glucanase，Induced gene expressionsystemic acquired resistance，SAR）是植物抵抗病原菌侵袭的复杂防御网络之一[1]。它是通过植物抗病基因与病原微生物的无毒基因相互识别和相互作用来实现的[2]。在植物与病原菌的互作中，植物产生防御反应并合成累积系列防御反应物质。其中病程相关蛋白(pathogenesis-related proteins，PR)的产生，是植物防御反应PR基因被诱导表达的结果。PR-2类蛋白具有β-1，3-葡聚糖酶活性，PR-3属于植物几丁质酶类。PR基因的诱导表达与植物的抗病性有关[3-5]。 甜菜是我国重要的糖料作物，是我国北方的主要经济作物之一。病害一直是影响甜菜产量和含糖率的重要因素，其中丛根病(Rhizomania)已成为当前甜菜生产上最为严重的病害之一。该病害是由甜菜坏死黄脉病毒(beet necrotic yellow vein virus，BNYVV)侵染，