石油污染土壤实验论文修改后.docVIP

丛枝菌根真菌-植物对石油污染土壤修复实验研究 王丽萍，郭光霞，华素兰，张玮玮 （中国矿业大学环境与测绘学院 江苏 徐州 221008） 摘要：本文模拟两种石油污染土壤浓度，采用Glomus mosseae(G.M)和Glomus versifome(G.V)，进行菌根真菌-植物对土壤石油烃降解研究。结果表明：石油污染浓度2000mg/kg和20000mg/kg条件下石油烃降解率与菌根侵染率玉米根干重植株干重呈现相关性0.8296~0.8785、0.9620~0.9745。接种丛枝菌根真菌处理的菌根侵染率、玉米生量和石油降解率均远高于对照处理丛枝菌根真菌促进石油降G.V真菌比G.M对高石油污染浓度具有更强的适应性，更适合作为处理高浓度石油污染的接种菌。双接种处理的菌根侵染率石油降解率均高于单接种处理两种菌种协同作用，植物生长和石油烃降解。 关键词：地表球囊霉双接种 侵染率 生量 石油烃降解率tudy on Bioremediation of Petroleum-Contaminated Soil with Arbuscular Mycorrhizal Fungi and Plant WANG Li-ping , GUO Guang-xia，HUA Su-lan，ZHANG Wei-wei （School of Environment and Spatial Informatics, China University of Mining Technology, Xuzhou, Jiangsu, 221008） Abstract：In order to quest a optimal method of mycorrhizal bioremediation on petroleum-contaminated soil, the effect of mycorrhizal fungi-plants on petroleum hydrocarbons degradation in soil was investigated under the condition of two simulated petroleum-contaminated soil concentrations with Glomus mosseae (G.M) and Glomus versifome (G.V) inoculated to corns by dual and single inoculation. The result shows that the degradation rate of petroleum hydrocarbon is significantly correlated with the mycorrhizal colonization rate, corn root dry weight and plant dry weight, the correlation coefficient is in the range of 0.8296~0.8785、0.9620~0.9745 with petroleum concentrations 2000 mg/kg and 20000 mg/kg, respectively. Mycorrhizal colonization rate, corn growth and degradation rate of petroleum hydrocarbons of samples inoculated with arbuscular mycorrhizal fungi are much higher than the control group without inoculation, which indicates that the arbuscular mycorrhizal fungi can enhance petroleum hydrocarbons degradation efficiently. G.V fungi is more adaptive and more suitable for high petroleum-contaminated soil remediation than G.M. The mycorrhizal colonization rate, degradation rate of petroleum hydrocarbons of the double inoculation treatments are higher than those of single inoculation ones，and the synergies effect be