bac-fish技术在植物基因组研究中的应用_论文网论文-by_gnksguybb（The application of _ thesis -by_gnksguybb BAC-FISH technique in the research of plant genome.）.docVIP

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2017-10-05 发布于河南
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bac-fish技术在植物基因组研究中的应用_论文网论文-by_gnksguybb（The application of _ thesis -by_gnksguybb BAC-FISH technique in the research of plant genome.）.doc

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bac-fish技术在植物基因组研究中的应用_论文网论文-by_gnksguybb（The application of _ thesis -by_gnksguybb BAC-FISH technique in the research of plant genome.）

bac-fish技术在植物基因组研究中的应用_论文网论文-by_gnksguybb（The application of _ thesis -by_gnksguybb BAC-FISH technique in the research of plant genome.） The application of _ BAC-FISH technique in the research of plant genomes This article will mainly discuss the principle of BAC-FISH technology. Comparative mapping study. Functional gene mapping. Keywords: BAC-FISH, karyotype analysis, comparative mapping, gene mapping In situ hybridization is a molecular cytogenetic technique that uses labeled DNA or RNA probes to locate specific target nucleic acid sequences directly at the chromosome, cell, or tissue levels. Its emergence marks the transition from traditional cytogenetic techniques to modern molecular genetic techniques. In situ hybridization has been developed greatly since its emergence. From radiolabeled to fluorescence labeling; from single probe to probe; chromosome preparation from traditional metaphase chromosomes and pachytene chromosomes, and then to the DNA fiber, the in situ hybridization technique has been greatly improved in the detection accuracy and sensitivity. However, due to the signal detection ability limit, or 5S and 45S rDNA, and other areas of telomere centromere tandem repeats up using the traditional technique of in situ hybridization, single or low copy small fragments of in situ hybridization is difficult to detect. Bacterial artificial chromosome fluorescence in situ hybridization (BAC-FISH) is a bacterial artificial chromosome (BAC) chromosome in situ hybridization clones as probes, due to exogenous clones is generally more than 100 kb, the probe and the target sequence of DNA the opportunity to meet, greatly improve the detection rate of hybridization signal. Therefore, it is a single copy or low copy DNA sequences of hybrid small fragments easier, and accurate and reliable, fundamentally overcomes the difficulties in single copy DNA hybrid network, greatly promote the plant especially on the diploid species genome, has now become the plant karyotyp