反相高效液相色谱法测定食品中的苏丹红1号（Determination of Sudan Red 1 in food by Reversed Phase High Performance Liquid Chromatography）.docVIP

反相高效液相色谱法测定食品中的苏丹红1号（Determination of Sudan Red 1 in food by Reversed Phase High Performance Liquid Chromatography） Determination of Sudan Red 1 in food by Reversed Phase High Performance Liquid Chromatography Gan yuan, Zhao Jian, Xiao Yifu, Wang Zhenghong, Gu Wanjiang [Abstract] Objective: to establish a reversed phase high performance liquid chromatographic method for the determination of Sudan Red 1 in food. Methods: Sudan 1 in food was extracted by ethanol A reversed-phase high performance liquid chromatography diode array detector (DAD) was used. Results: the linear range of this method ranged from 0.5 to 1000 fig / M1, and the standard deviation was 0.18, The relative standard deviation was 0.73%, the average recovery was 95.43%, the minimum detection limit was 0.2 ng, and the lowest detectable concentration was 0.02 mg / kg. Synthetic pigments (lemon) Lemon yellow, sunset yellow, carmine, amaranth, blue, indigo and indigo) did not affect the determination of. Conclusion: this method has a wide range of linearity, high precision and good accuracy, The selectivity is strong and can satisfy the qualitative and quantitative analysis of Sudan 1 in food. Keywords reversed-phase high performance liquid chromatography; food; Sudan Red 1; [CLC number] R122.7 [document identification code]A [article number] 1003 - 8507 (2005) 12 - 1760 - 02 TO Dj: THE SUDAN I, IN FOOD USING METHOD OF RE HI LC.GAN Yuan ZHAO, Jian, XIAO Yi, et a1. Centrofor, Disease, Controland, Prevention, ofChongqing, Gty, Chongqing, 400042. Abstaet:Objective:Toevaluatethemethxt, of, detection, of, Sudan, I, infoodsbased, OI3.theuseof, RE - HPU.Methods:Sudan The in foods were detected by re-HPLC DAD after extracted by alcoho1.Results:This method could detect Sudan I in samples EF- Fectlvdy, while, its, eoneentrat~on, va.~ed, from, 0.5~g / RNL, to, I-tg / m1.The,, Stan, dard, deviation,, WaS, 0.18.relative, standard, devia- Tion was 0.73%, an, D, average, recovery, 94.43%.0.2, ng, was, observed, as, its