微流控芯片在细胞分选中的分析技术进展-中国细胞生物学学报.PDF

中国细胞生物学学报 Chinese Journal of Cell Biology 2013, 35(5): 727–733 微流控芯片在细胞分选中的分析技术进展 1 2 1 1 3 3 3 3* 刘 琳 厉坤鹏 胡志敏 董军磊 欧 元 李彩霞 赵兴春 叶 健 1 2 3 ( 中国人民公安大学, 北京 100038; 上海市第一人民医院, 上海 200080; 公安部物证鉴定中心, 北京 100038) 摘要 微流控芯片分析技术以其快速、高效、高能量、低消耗、集成化和微型化等特点在 多个研究领域发展非常迅速。该文根据分选原理不同, 将微流控芯片上的细胞分选方法分为主动 式细胞分选与被动式细胞分选, 从这两方面总结了目前微芯片分选细胞的进展, 并对该技术在细胞 分选中的应用前景作了进一步的展望。 关键词 微流控芯片; 细胞分选; 细胞分离 Progress in Analytical Techniques of Microfluidic Chip on Cell Sorting 1 2 1 1 3 3 3 3 * Liu Lin , Li Kunpeng , Hu Zhimin , Dong Junlei , Ou Yuan , Li Caixia , Zhao Xingchun , Ye Jian 1 2 ( People’s Public Security University of China, Beijing 100038, China ; Shanghai First People’s Hospital, Shanghai 200080, 3 China; Institute of Forensic Science Ministry of Public Security, Beijing 100038, China) Abstract Microfluidic chip techniques develop very fast owing to it’s high rate, high efficiency, high power, low reagent cost, integrating and miniaturization in many research fields. Based on the operating principles, the various cell sorting methods on microfluidic chip are broadly categorized as active or passive techniques in this article. Recent research progress of microfluidic chip techniques on cell sorting are summarized mainly in the two aspects. In addition, the application prospect of microfluidic chip on cell sorting is also briefly discussed. Key words microfluidic chip; cell sorting;