斑马鱼不同发育时期血管内皮生长因子受体2基因表达的实时定量PCR分析.pdfVIP

ofAnhui 安徽农业科学。Journal Am．Sci．2010。38(16)：8444—8445，8454 责任编辑马树梅责任校对卢瑶 斑马鱼不同发育时期血管内皮生长因子受体2基因表达的实时定量PCR 分析 孙桂金啦，潘杰1，刘可春孙，王雪2，王思锋2 (I．山东师范大学生命科学学院，山东济南250014；2．山东省科学院生物研究所，山东济南250014) h的斑 h有所下降。12h表达量最低，72h表达量最高，与其他发育期均有显著差异，、[结论]斑马鱼血 表达量在12．72h呈上升趋势，在96 管发育至72h达成熟阶段血管发育成熟前，VEGFR一2基因表达水平逐步增长；血管发育成熟后，VEGFR一2基因表达水平下降。 关键词宽马鱼；实时定量PCR；血管内皮生长因子受体2 中图分类号Q95—33文献标识码A 文章编号0517—661l(20LO)16—08444—02 PCR ofVascularEndothelialGrowthFactor atDifferent Sta- Real．timeQuantitative Receptor-2Expression Developmental Analysis ofZebrafish ges SUN et oflife Normal 250014) Gui-jin sciences，ShandongUniversity，Jinan，Shandong al(College researchaimedto the ofvascularendothelial zebrafishindif- Abstract『Objective]Theinvestigateexpression growthfactor-2(VEGF-2)in 1arvae．Real—time ferent T0talRNAswereextractedfroml and96hzebrafish and developmentalsrages．『Method1 2．24，48，72 embryos examinethe of陋G职-2．111edatawere RT．PCRwas to level quantitative performed expression analyzedby levelofVEGFR一2increased froml2hto72hand decreasedat96h．The levelwas10west expression gene gradually subsequently expression atl2hand at72 differencewiththatofother levelofVEGFR一 highesth．withsignificant developmentalstages．[Conclusion]Theexpression 2increased beforevesselmaturationanddecreasedasvesselsmature． gradually words Key Zebr幽sh：Real—timequantitativePCR；VEGFR-2 斑马鱼是一种小型热带淡水鱼，已成为继果蝇、线虫、小 1．2．3 白鼠之后的又一模式生物体。斑