离子交换吸附分离茶氨酸的研究-食品科学.PDFVIP

148 2007, Vol. 28, No. 09 食品科学 ※工艺技术 1 2, 1 1 朱 松 ，王洪新 *，陈尚卫 ，戴 军 (1.江南大学分析测试中心，江苏 无锡 214122； 2.食品科学与安全教育部重点实验室，江南大学食品学院，江苏 无锡 214122) ：本实验以目前工业上生产茶多酚后的废茶水为原料，通过超滤、脱色等预处理后，通过离子交换树脂 对茶氨酸进行吸附分离，探索从其中分离制备茶氨酸的工艺。选用001 ×7 阳离子交换树脂，分析了影响茶氨酸 交换容量的因素，并对树脂吸附茶氨酸的动力学进行了研究。通过响应面分析法确定固定床离子交换工艺中合适的 吸附工艺条件为：pH 值为 3.4，上样液浓度为 3.0mg/ml，上样流速为1.7 柱床体积/h。最后确定洗脱工艺条件， 用pH11.3 的氨水洗脱后得到茶氨酸含量为58%，离子交换过程中茶氨酸的回收率为82%。 ：茶氨酸；离子交换；吸附；分离；响应面分析 Study on Adsorption and Separation of Theanine by Ion Exchange 1 2, 1 1 ZHU Song ，WANG Hong-xin *，CHEN Shang-wei ，DAI Jun (1.Testing and Analysis Center, Southern Yangtze University, Wuxi 214122, China；2.Key Laboratory of Food Science and Safety, Ministry of Education, School of Food Science and Technology, Southern Yangtze University, Wuxi 214122, China) Abstract ：The technology of isolation and purification of theanine extracted from the spent tea water after the tea polyphenols had been removed was studied. Adsorption and separation of theanine by ion exchange after being pretreated by ultrafiltration and decolorization. 001×7 resin was adopted as the adsorption medium of ion exchange chromatography to separate theanine from the pretreated liquid. The factors affected the exchange capacity of theanine were assayed, and the kinetics of adsorption also studied. By response surface analysis (RSA) the optimal conditions of adsorption in the fixed-bed of 001×7 resin are resulted as follows: pH of pretreatment liquid 3.4, concentration of theanine 3.0 mg/ml, and sample flow rate 1.7 BV/h. The crude theanine with 58% purity is obtained t