Shh对发育中小鼠视觉传导通路的影响.pdfVIP

2006;26(12) 南方医科大学学报(J South Med Univ) 1679 # # Changes of retinofugal pathway development in mouse embryos after Sonic hedgehog antibody perturbation 1 2 3 HAO Yan-li , CHAN Sun-on , DONG Wei-ren 1 2 Department of Anatomy, Guangzhou Medical College, Guangzhou 510180, China; Department of Anatomy, Chinese University of Hong Kong, Hong Kong, China; 3Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China Abstract: Objective To understand the function of Sonic hedgehog in chiasm development in mouse embryos of embryonic day 13 (E 13) to E 15. Methods Brain slices of E 13-E 15 mouse embryos containing the optic pathway from the eyes to the optic tract were prepared and cultured in DMEM/F 12 in the presence of 10% fetal bovine serum at 37 ! in a rolling incubator for 5 h. The antibody to Shh was added into the culture medium of the slices in the treatment group, while no additional chemical or only normal mouse IgG was added in the control groups. After culture, the brain slices were fixed and a DiI granule was inserted into the optic disc in one eye. Seven days later, the tissue overlying the chiasm was removed to expose the DiI-labeled chiasm for observation under confocal microscope, and the images were analyzed by METAMORPH software. Results Shh antibody treatment produced a reduction of crossing of the earliest reti