以斑马鱼为模式动物研究器官的发育与再生.pdfVIP

HEREDITAS (Beijing) 2013 4 , 35(4): 421―432 ISSN 0253-9772 综 述 DOI: 10.3724/SP.J.1005.2013.00421 以斑马鱼为模式动物研究器官的发育与再生 李礼, 罗凌飞 , 400715 斑马鱼因其受精卵体外发育、胚胎透明、具有较强的再生能力以及适于大规模遗传筛选的优势, 成为研 究脊椎动物器官发育与再生的新兴模式动物。通过数十年的探索, 科研工作者已经在斑马鱼中建立了一套成熟 的研究方法, 并对斑马鱼胚胎发育早期的细胞命运决定和分化、组织器官的形态建成以及受损后的再生过程有 了初步的认识。近年来, 随着遗传筛选技术的大规模开展和活体成像技术在斑马鱼中的深入应用, 许多在小鼠 等模式动物中悬而未决的问题开始得到充分解答。随着研究的不断深化和技术的不断更新, 以斑马鱼为模式动 物, 对脊椎动物器官发育与再生的研究将会更加深入, 相关的调控机制也会被逐步探明, 从而为临床相关疾病 的防治提供富有价值的参考。文章通过对近年来发表的文章进行回顾, 总结了斑马鱼作为模式动物研究中枢神 经系统、肝脏和胰腺、血液细胞和血管等重要器官早期发育过程及其调控机制的进展, 并阐述了以斑马鱼研究 尾鳍、心脏、肝脏等器官再生的优势和初步发现。 斑马鱼; 发育; 再生 Zebrafish as the model system to study organogenesis and regenera- tion LI Li, LUO Ling-Fei School of Life Sciences, Southwest University, Chongqing 400715, China Abstract: Zebrafish (Danio rerio) is becoming more and more popular as a model organism to study vertebrate organo- genesis and regeneration, taking the advantage of its extra-uterine development, transparency, strong regeneration ability and suitability for the large scale genetic screen. In the past decades, a series of useful methods have been established in zebrafish, which allows studies of important questions in developmental biology including cell fate determination, differen- tiation, morphogenesis, and regeneration after tissue/organ injury. Recently, using large scale mutagenesis screen and im- proved live imaging techniques, many questions that had not been well understood in mice have been successfully investi- gated in zebrafish. Therefore, we are confident that the zebrafish model system will further help decipher the cellular and molecul