锌指核酸酶的构建及其在动物基因组编辑中的应用.pdf

  Classification code: Q783.1 University code: 10712 UDC: 575 Postgraduate number: 2010060147 Confidentiality level: Unclassified       Dissertation for Doctor Degree Northwest A ＆F University in 2013         CONSTRUCTION OF ZINC FINGER NUCLEASES AND ITS APPLICATION OF ANIMAL GENOMIC EDITING                                            Major: Genetics Research field: Animal Genetics Name of Postgraduate: Wang Ling Adviser: Professor Zhang Zhiying Co-adviser: Date of submitted: August, 2013       Yangling Shaanxi China     本研究由 转基因新品种培育重大专项课题研究计划（2009Z 和国家自然科学基金提供资助 This study was supported by grants from The research plan of grand specialized topics for cultivation of new transgenetic biological varieties (2009Z and the National Natural Science Foundation of China     锌指核酸酶的构建及其在动物基因组编辑中的应用 摘 要 精确高效的实现内源基因靶向编辑是转基因技术的难点和重点。传统的自发同源重 组方法的效率低下和逆病毒载体法的安全性差等问题，限制其广泛应用和转基因新品种 的培育。锌指核酸酶作