红曲霉中麦角固醇的培养条件优化无机盐.docVIP

第 PAGE 30页 贵州大学本科毕业论文（设计） 红曲霉中麦角固醇的培养条件优化——无机盐 摘 要 红曲霉(monascus)是我国最早应用于食品加工的有益真菌之一，主要集中应用在传统酒曲、制醋、着色、防腐等领域，国外红曲霉主要应用于肉品加工及其他食品着色方面。红曲霉可产生淀粉酶、糖化酶、蛋白酶、酯化酶、果胶酯酶、麦芽糖酶等酶类；其药理活性物质可抑制胆固醇活性、降血压物质——麦角固醇。 目前麦角固醇的生产主要采用微生物发酵法，关于碳源、氮源、初始pH值、培养时间、通气量等因素对细胞中麦角固醇的含量研究，有很多人在这方面进行了深入而透彻的研究。而无机盐对红曲霉菌产麦角固醇的影响国内外尚未??过报道（就目前而言；仅有惠丰立等人的《无机盐对酵母菌产麦角固醇的影响》[13]一文的发表。本文在原有工作的基础上，扩大了无机盐对红曲霉菌产麦角固醇的影响筛选范围。 其主要研究了Ca2+、Mg2+、Fe2+ 、K+和Na+ 对红曲霉细胞生长及麦角固醇合成的影响。结果表明：这几种无机盐在一定的浓度范围内均能促进或抑制红曲霉细胞生长。提高或降低生物量，对麦角固醇产量的影响明显的差别。当培养基中分别加入一定量的Ca2+、Mg2+、Fe2+ 、K+和Na+ 时，Ca2+、Mg2+刺激麦角固醇合成作用最强，其中Mg2+对麦角固醇单位产量影响最为显著高到21.75mg/g。又当接种量为10mL的种子液，培养温度30 0C 、转速为200r/min摇床培养120h。在此条件下，ZZ红曲霉菌株产麦角固醇单位含量达24.48mg/g干菌体.麦角固醇总重46.52mg 关键词:红曲霉，麦角固醇（Ergosterol）, 液体发酵，无机源，紫外分光光度法。 ABSTRACT The monascus is our country most early applies oneof to food processing beneficial fungi, mainly concentrates theapplication in traditional domain and so on liquor tune, systemvinegar, coloration, anticorrosion, the overseas red tune mildewmainly applies to the meat processing and other food colorationaspects. The monascus produces enzyme class and so on amylase,saccharification enzyme, proteinase, esterification enzyme, pectinesterase, maltose; Its pharmacology active material may suppress thecholesterol activeness, fall the blood pressure matter - ergosterol. At present the ergosterol production mainly uses the microorganismzymotechnics，About the initial pH value, the raise time, passfactor and so on spirit to the cell in the ergosterol contentresearch, had very many people to conduct thorough and the thoroughresearch in this aspect. But the inorganic salt becomes lucky the tunemold domestic and foreign to produce the ergosterol the influence notyet to see the report (in the present case; Only has Huifengliperson Inorganic salt To produce Ergosterol toSaccharomycetes Infl