滋肾活血方在抗肾纤维化中对HA, LN, TGF-β1 干预作用的实验研究.pdfVIP

zy@cjocm.cn 中医临床研究 010 CJCM 中医临床研究 2013 年第 5 卷 第 9 期 -1- 滋肾活血方在抗肾纤维化中对HA、LN、TGF-β1 干预作用的实验研究 An experimental study of intervention effect of the Zishen Huoxue recipe against HA, LN, TGF-β1 in anti-renal fibrosis 曹秋彩 （河南省郑州市第九人民医院，河南 郑州，450000 ） 中图分类号：R692.3+1 文献标识码：A 文章编号：1674-7860 （2013 ）09-0001-03 证型：IBD 【摘 要】 目的：探讨滋肾活血方对慢性肾病肾纤维化的防治作用及机制。方法：采用右侧肾切除加重复尾静脉注射阿霉素 制备肾纤维化大鼠模型[1] 。设滋肾活血方治疗组、卡托普利对照组、模型组、空白组。8 周后观察各组大鼠 24h 蛋白定量、血清尿 素氮（Bun ）、肌肝（Scr ）、透明质酸（HA ）、层粘蛋白（LN ）和肾组织中转化生长因子 β1 （TGF-β1 ）表达的变化。结果：滋肾 活血方能够减少阿霉素肾纤维化大鼠尿蛋白的排泄，降低血清 Bun 、Scr、HA 、LN 的含量，抑制肾组织中 TGF-β1 的过度表达。 结论：滋肾活血方能够延缓肾纤维化的进程，具有保护肾功能的作用。 【关键词】滋肾活血方；肾纤维化；TGF-β1；HA；LN 【Abstract 】 Objective: To investigate the effect of Zishen Huoxue recipe anti-chronicity nephropathy renal fibrosis preventive and therapeutic effect and mechanism. Methods: According to reference documents[1], we reproduced the rat model of adriamycin nephrosis by having right nephrectomy adding to injecting repeatedly ADR through the vein of tail. The rats were divided into four groups for the bland contral group, the Zishen Huoxue recipe group, the captopril group and the model group. We observe quantity of 24h urinary protein excretion, blood urea nitrogen and creatinine, HA , laminin and tranforming growth factor β1 in kidney tissues after 8 weeks. Results: The Zishen Huoxue recipe can decrease quantity of 24h urinary protein excretion, degrade blood serum hyaluronic acid and laminin contents, depress tranforming growth factor β1 over expression in kidney tissues. Conclusion: Zishen Huoxue recipe can prevent process of the renal fibrosis, to have preserve effect of the renal function. 【Keywords 】 Zishen Huoxue recipe; Renal fibrosis; TGF-β1; HA; LN doi:10.3969/j.issn.1674-7860.2013.09.001 肾纤维化（包括肾小球硬化和小管间质纤维化）是指肾