水稻叶绿体的petG-trnP 片段的测定和系统学分析.pdfVIP

中国农学通报 2010,26(23):81-84 Chinese Agricultural Science Bulletin 水稻叶绿体的petG-trnP片段的测定和系统学分析 刘志文，韩 旭 （大连工业大学生物工程学院，大连116034） 摘 要：本研究主要是对水稻的叶绿体p etG-trnP 片段进行PCR 直接测序和序列结构的分析。结果表明， 水稻的p etG-trnP 的序列十分保守，其间隔区全长为316 bp ，扣除trnW 序列后的非编码区长为242 bp ，其 中两端非编码区分别为116 bp 和126 bp 。非编码区中G+C 含量在37.19%~38.02%之间。对5 种水稻材 料进行ClustalW 等分析表明，它们的序列结构有所变异，共有7 个变异位点，比例为2.89% ，这预示着水 稻的p etG-trnP 的非编码区中有着一定的系统学信息，可在分子水平上为稻属的系统关系提供一定的证 据。 关键词：水稻；叶绿体p etG-trnP ；系统学 中图分类号：S511 文献标志码：A 论文编号：2010-2391 TheSequencingandPhylogeneticsAnalysisoftheRiceChloroplastDNApetG-trnPFragment Liu Zhiwen, Han Xu (College of Bioengineering , Dalian Polytechnic University , Dalian 116034) Abstract: The rice chloroplast petG-trnP fragment was sequenced and analyzed in this study. The results showed that the petG-trnP intergenic spacer was very conservative, and its length was 316 bp. The length of non-coding region was 242 bp deducting the trnW sequence, and the two ends of the non-coding region were 116 bp and 126 bp, respectively. The G+C content ranged from 37.19% to 38.02% in the non-coding region. The ClustalW analysis showed that the petG-trnP fragment structure was variable, and there were 7 variable sites (2.89% ) in the non-coding region. The results indicated that the non-coding region of petG-trnP contained some phylogenetic information, and could provide the direct molecular evidence to clarify the Oryza species origin and evolution. Keywords:rice; chloroplast petG-trnP; phylogenetics 0 引言 体组栽培稻和野生稻以及和一年生杂草稻等之间的亲 [2-4] 稻属（Oryza L.）是与人类生活最为密切相关的禾 缘关系，缺乏分子水平上的系统学研究 。 本科