陡脉冲电场生物学效应的试验研究-第三军医大学学报.DOCVIP

陡脉冲电场生物学效应的实验研究 顾华妍1，熊正爱1，李成祥2，向鹏2，姚成果2，孙才新 2，吴静1，杨晓燕 （1重庆医科大学第二临床学院妇产科，重庆400010；2重庆大学高电压与电工新技术教育部重点实验室，重庆400044） 　摘要: 目的　研究陡脉冲电场生物学效应。方法 建立30只新西兰大白兔VX2移植性肝癌模型,监测陡脉冲作用前后同一部位肿瘤组织的温度变化，取作用后肿瘤组织送光镜、电镜检查。结果 陡脉冲电场覆盖区组织温度升高不明显，未处理前温度为（34.8±2.07）℃，处理后电极针处温度为（35.93±2.19）℃，脉冲作用前后，温度变化差异无统计学意义(P0.05)。HE染色显示：脉冲作用活体兔肝VX2肿瘤组织后，引起肿瘤组织凝固性坏死。电镜结果显示肿瘤细胞受到不可逆性损伤。结论　陡脉冲可致肿瘤细胞发生不可逆性损伤，其对组织的损伤是一种非热效应，并非传统意义上的热疗。 关键词:脉冲；VX2肿瘤；电热效应 Study On The Bioeffects Of steep pulse GU Huayan1 XIONG Zheng-ai1 LI Cheng-xiang2 XIANG Peng2 YAO Chen-guo2 Sun Caixin2 WU Jing1 YANG Xiaoyan1（1.Department of Gynaecology and Obstetrics ,The Second Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400010; 2Key Lab of High Voltage Engineering and Electrical New Technology ,Ministry of Education,Chongqing 400044,China.） ABSTRACT: objective To investigate the bioeffects of steep pulse. Methods Rabbits with VX2 liver tumor were divided into four treatment groups and control group．Every rabbit was exposed to steep pulse electric field with serial voltage dependently, The temperature of the injuring tissues was measured. histological examination were used to observe the injuring effect, and ultrastructural changes were detected. changes in the tumors volume were observed 3 days after the treatment．Comparison was made between the four treatment groups and control group，and within the four groups.Results No significant temperature rise was detected（P0.05）. After treatment, pyknosis, karyoclasis and karyolysis of tumor cells were observed light and electron mircroscope.Serious changes appeared more time later。 Conclusion Heat-effect didn’t play important rloe in SP therapy. Steep pulsed electric fields can kill the tumor cell and damaged the tumor . Key words：Pulse;VX2; Electrocaloric effect? 生物学系统(生物大分子、细胞、组织、器官、生物体等)对电磁波(场)所产生的与生命现象(生理、生化、结构、功能等)有关的响应称为电磁波的生物学效应[1] 。通常,可将其分为热效应和非热效应。所谓电磁波的生物学热效应,是指由进入生物系统内的电磁波转变来的焦耳热导致的温度变化所引起的生物学效应;所谓电磁波的生物学非热效应,是指与温度变化无关的、由进入生物系统的电磁波直接