刺五加多糖对Lewis荷瘤小鼠抗肿瘤免疫调节作用及-中国水稻科学.PDF

周丽菁等摇 刺五加多糖对Lewis荷瘤小鼠抗肿瘤免疫调节作用及机制的研究 摇 第6期 ·849 · doi:10.3969/ j.issn.1000鄄484X.2017.06.009 ·中医中药与免疫 · 刺五加多糖对Lewis荷瘤小鼠抗肿瘤免疫调节作用及机制的研究淤 周丽菁摇 龙婷婷摇 周摇 星摇 鲍依稀摇 (重庆医科大学附属第二医院检验科,重庆 400010) 摇 摇 中图分类号摇 R285郾5摇 R392郾5摇 摇 文献标志码摇 A摇 摇 文章编号摇 1000鄄484X(2017)06鄄0849鄄06 [摘摇 要]摇 目的:探讨刺五加多糖(ASPS)对Lewis荷瘤小鼠TLR4 信号转导通路的调控机制。 方法:用 C57BL/ 6建立 Lewis实体型荷瘤小鼠模型,随机分为生理盐水组(NS组)、阿霉素组(ADM组)和ASPS低、中、高剂量组,灌胃给药25 d后,称 重计算瘤重、抑瘤率、胸腺指数和脾脏指数。 收集荷瘤小鼠眼球血,ELISA检测外周血中细胞因子TNF鄄琢、IL鄄1茁、IL鄄6 和IL鄄 12p70 的分泌情况。 采用实时荧光定量 PCR(Q鄄PCR)和Western blot 法分别检测荷瘤小鼠脾细胞中TLR4 相关节点TLR4、 MyD88、TRAM、TRAF6、NF鄄资B p65、AP鄄1基因和蛋白的表达水平。 结果:与NS组相比,ASPS 中剂量组荷瘤小鼠的瘤重明显降 低,抑瘤率和免疫器官指数显著升高(P0郾05)。 与NS组相比,ASPS显著促进荷瘤小鼠外周血TNF鄄琢、IL鄄1茁和IL鄄6 的分泌 (P0郾05),对IL鄄12p70 的产生无显著影响(P0郾05)。 ASPS显著上调TLR4 信号通路中TLR4、MyD88、TRAF6、NF鄄资B p65、 AP鄄1基因和蛋白在荷瘤小鼠脾脏中的表达(P0郾05),而对TRAM无显著作用(P0郾05)。 结论:TLR4 信号通路可能是ASPS 在Lewis荷瘤小鼠体内发挥免疫调节及抑制肿瘤作用的通路之一。 [关键词]摇 刺五加多糖;肺腺癌;免疫调节;TLR4信号通路 Immunomodulatory effects of Acanthopanax Senticosus polysaccharides on lewis tumor鄄bearing mice through TLR4 signaling pathway ZHOULi鄄Jing,LONG Ting鄄Ting,ZHOUXing,BAO Yi鄄Xi郾Department of Clinical Laboratory,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China [Abstract]摇 Objective:To investigate the effects of Acanthopanax Senticosus polysaccharides (ASPS) on TLR4 signaling pathway in Lewistumor鄄bearing mice,andto explorethe immunomodulatory mechanism of ASPS郾Methods:Lewislung carcinoma cells and C57BL/ 6 mice were used to establish the animal model for solid tumor郾The tumor鄄bearing mice were divided into five groups: normal saline (NS)group,Adriamycin (ADM)group,ASPSlow鄄dosegroup,ASPSmiddle鄄dosegroupandASPShigh鄄dosegroup郾After 25 d treatment,theweight of tumor,tumor inhibition rate and immune organ index were measured郾ELISA were applied to detected the cytokines in peripheral blood of tumor鄄bearing mice郾Quantitative real鄄time PCR (Q鄄PCR) and Western blot were selectively