分离与分析细胞壁组成重要.pdfVIP

Analytical Biochemistry 421 (2012) 657–666 Contents lists available at SciVerse ScienceDirect Analytical Biochemistry journal homepage: www.else /locate/yabio Isolation and analysis of cell wall components from Streptococcus pneumoniae Nhat Khai Bui a,1, Alice Eberhardt a,1,2, Daniela Vollmer a, Thomas Kern b,3, Catherine Bougault b, Alexander Tomasz c, Jean-Pierre Simorre b, Waldemar Vollmer a,⇑ a Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4AX, UK b Institut de Biologie Structurale, UMR5075 (CEA/CNRS/UJF), 38027 Grenoble, France c Laboratory of Microbiology, Rockefeller University, New York, NY 10021, USA a r t i c l e i n f o a b s t r a c t Article history: The complex and heterogeneous cell wall of the pathogenic bacterium Streptococcus pneumoniae is com- Received 23 September 2011 posed of peptidoglycan and a covalently attached wall teichoic acid. The net-like peptidoglycan is formed Received in revised form 14 November 2011 by glycan chains that are crosslinked by short peptides. We have developed a method to purify the glycan Accepted 22 November 2011 chains, and we show that they are longer than approximately 25 disaccharide units. From purified pep- Available online 1 December 2011 tidoglycan, we released 50 muropeptides that differ in the length of their peptides (tri-, tetra-, or penta- peptides with or without mono- or dipeptide bra