甘草鲨烯合酶1基因多态性及其与β-香树脂醇合成酶共表达对β-香树脂醇积累的影响研究.pdfVIP

·734 · 药学学报 ActaPharmaceuticaSinica2014，49(5)：734—741 Enhancingtheaccumulationoffl-amyrininSaccharomyces cerevisiaebyCO—expressionof rrhizauralensissqualene synthase1andp-amyrinsynthasegenes LIU Ying，CHEN Hong—hao，W EN Hao，GAOYa，WANG Li—qiang，LIU Chun—sheng (SchoolofChinesePharmacy,BeoingUniversityofChineseMedicine，Bejiing100102，China) Abstract：GlyeyrrhizauralensisFisch．exDC iswidelyusedintraditionalChinesemedicine(TCM)． Among itsvariousactivecomponents，glycyrrhizicacid isbelievedtobethemarkercomponent． Squalene synthase(SQS)and amyrinsynthase 一AS)arekeyenzymesinthebiosyntheticpathwayofglycyrrhizic acidinG uralensis．Torevealtheeffectsofco-expressionofSQS1andB—ASgenesonthispathway,7yeast expressionvectorsharboringdifferentSQS1variantsandBASwereconstructedandexpressedinSaccharomyces cerevisiaeasfusionproteins．TLCandGC—MSresultsshowedthatco-expressionofSQS1andASenhanced theaccumulationofB-amyrin．TheeffectsofSQS12weremoreobviousthantheothertwoSQS1variants． Thisstudy issignificantforufrtherinvestigationsconcernedwith exploringthebiosny thesisofglycyrrhizic acid invitroandstrengtheningtheefficacyofG uralensisbymeansofincreasingthecontentofglycyrrhizic acid． Keywords：Glyeyrrhizauralensis；squalenesynthase；B--amyrinsynthase；co·e·xpression；genepolymorphism； glycyrrhizicacid；Saccharomycescerevisiae CLCnumber：R931 Documentcode：A ArticleID：0513-4870(2014)05—0734—08 甘草鲨烯合酶 1基因多态性及其与 ．香树脂醇合成酶 - H- ， 、 表达对 J67．香树脂醇积累的影响研究 刘 颖，陈宏昊，文 浩，高 雅，王礼强，刘春生 (北京中医药大学中药学院，北京 100102) 摘要：甘草是我国最常用的大宗药材之一。甘草中最主要的活性成分为甘草酸，其生物合成途径受到很多酶 的调节。鲨烯合酶 (squalenesynthase，SQS)及 香树脂醇合成酶 (amyrinsynthase， AS)是其中的两种关键 酶。为了揭示鲨烯合酶基因多态性及其与 ．香树脂醇合成酶共表达对MVA代谢途径的影响，本文构建了7种载