大学分子的生物学经典双语课件Chapter5transcription.ppt

esterification [e?sterifikei??n] n. 酯化作用 Splicing occurs in two stages. First the 5 exon is cleaved off; then it is joined to the 3 exon Nuclear splicing occurs by two trans-esterification reactions in which an OH group attacks a phosphodiester bond 过程 Splicing requires the 5’ and 3’ splice sites and a branch site just upstream of the 3 splice site The branch site is a short sequence just before the end of an intron at which the lariat intermediate is formed in splicing by joining the 5’ nucleotide of the intron to the 2’ position of an Adenosine A lariat is formed when the intron is