人参根皂苷及茎叶皂苷对孤独饲养小鼠自发行为及-第三军医大学学报.docVIP

人参根和茎叶皂苷对孤独饲养小鼠自发运动及攻击行为的影响 姚丽娜 ，张丽杰，姜成忠 （哈药集团制药六厂 150056） 摘 要 目的 研究人参根和茎叶皂苷对孤独饲养小鼠自发运动及攻击行为的影响。方法 小鼠孤独饲养四周后，分别以人参根及茎叶皂苷溶液灌胃，按高剂量100mg/kg，低剂量25mg/kg给药，并以生理盐水为空白对照，观察两者对小鼠自发运动及攻击行为的影响。结果 人参根皂苷可减少小鼠的自发运动及攻击行为（p0.01），人参茎叶皂苷此作用不明显。结论 人参根皂苷具中枢镇静的功效，而人参茎叶皂苷作用相反。 关键词 人参根皂苷 人参茎叶皂苷 孤独饲养 自发运动 攻击行为 Study on the Effects of Ginsenosides from Ginseng Root or from Ginseng Stem and Leaf on Spontaneous movement and aggressive behaviour of Isolated Mice YAO Lina，ZHANG Lijie，JIANG Chengzhong （The Sixth Factory of Harbin Rharmaceutical Group 150056） Abstract：Objective：This article is focused on the effects of ginsenosides from ginseng root or from ginseng stem and leaf on spontaneous movement and aggressive behaviour of isolated mice. Methods：Four weeks of isolated mice was administered intragastrically with ginsenosides solution from ginseng root or from ginseng stem and leaf respectively at a high dosage of 100mg/kg，and a low dosage of 25mg/kg. 0.9% sodium chloride solution was taken as blank control group. Then the effects of these two kinds of ginsenosides solution were studied on spontaneous movement and aggressive behaviour of isolated mice. Results：The spontaneous movement and aggressive behaviour of isolated mice were obviously reduced by administration with ginsenosides solution from ginseng root，not from stem and leaf. Conclusions：Ginsenosides solution from ginseng root, not from stem and leaf，has central sedative effect. Key word：ginsenosides from ginseng root；ginsenosides from ginseng stem and leaf；isolated mice； spontaneous movement；aggressive behaviour 随着社会的进步、发展，人们的生活压力越来越大，用脑过度精神长时间紧张，容易造成植物神经功能紊乱，失眠、多梦、扰乱了人们的正常生活。多是通过抑制中枢神经、强制催眠来发挥作用，长期服用Open field实验箱（透明有机玻璃制成，长50cm，宽40cm，高25cm，内部为10cm×10cm的方格）。 1.4 统计方法 应用spss8.0统计软件，实验数据以（￣X±s）表示，经方差分析后采用t检验进行检定。 2 结果 2.1 孤独饲养对小鼠自发运动量及攻击行为的影响 适应性群居饲养一周后，将小鼠随机分成6组，5组孤独饲养，每隔15天，观察其自发运动量的变化及咬架次数。统计结果：第15天时,小鼠自发运动及咬架次数t=5.25，P0.01，t=8.71,P0.01；第30天时，t=9.04，P0.01，t=14.17,P0.01；可见，小鼠自发运动量随孤独饲养周期延长而明显增加，统计学处理具显著性意义。 表1 孤独饲养对小鼠自发运