基于串级质谱信息进行蛋白质数据库搜索的结果可靠性分析-分析化学.PDFVIP

37 ( FENXIHUAXUE) 10 2009 10 Ch inese Journal of Analytical Chem istry 1473~ 1478 董乃平 李洪东 梁逸曾* (, 410083) SEQUEST M ascot M ascot SEQUEST , , M ascot SEQUEST , ; M ascot SEQUEST , SEQUEST M ascot, , Decoy , , , Decoy 1 , [ 1~ 3] , ( LC), , , ( Collision Induced D issoc-i ation, CID)(M S/M S), M S/M S, , , LC- M S /M S, [ 4] 3: ( PeptideM ass F ingerprint, PMF) , M S /M S, MS /M S [ 5] , , M S/M S [ ] [ 7] [ 8] [ 9] [ 10] SEQUEST , M ascot , X! tandem , OM SSA , Phenyx M S/M S, [ 11] [ 12] , , , , , [ 13, 14 ] [ 15] , , MS /M S [ 1 , 17] , [ 18] , , , , M ascot SEQUEST, Lasso( Least absolute shrink- [ 19] [ 20] age and selection operator) , -( PLS-LDA ) ( SVM ) , M ascot SEQUEST ; , ( Decoy)M ascot( FDR) 2 2. 1 http: / / regis-w eb. systemsbiology. net/PublicDatasets/[ 21] 18 2009-01-04; 2009-05-15 (N o.( Nos. 200 DFA41090, 2007DA40 80) * E-m ail: y izeng_liang@ 2 3. net 37 1474 8 LC-M S/M S , ( ( L inear ion traps) 3D ( 3D Ion traps)QTOF( Quadrupole tmi e-of-flight)MALD I-TOF-TOF, 3D LC-M S/M S (Thermo Electron LCQ DECA ) [ 21] SEQUEST K lmi ekH. influenzae 10 0700, 15247 M ascot(www. m atrixscience. com ) LC-M S/M S Sw iss-Prot ( Sw issProt