香得蕉果皮黄酮-丙酸复配剂的毒理学研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2015, Vol.31, No.7 香蕉果皮黄酮-丙酸复配剂的毒理学研究 顾采琴，温小龙，朱冬雪，刘鹏，赖雅平，林金莺，曾庆祝 （广州大学化学化工学院食品系，广东广州510006） 摘要：本文采取微波提取法从香蕉皮中提取得到黄酮粗提物，利用H103 大孔树脂对提取物进行纯化，与丙酸复配得到复配剂。 以香蕉果皮黄酮-丙酸复配剂为受试物，根据国家卫生部发布的《食品安全性毒理学评价程序和方法》对其进行毒理学试验，以便在 生产实践中推广应该。利用小鼠为试验对象，进行了急性毒性试验（LD50 ）、骨髓细胞微核试验、小鼠精子畸形试验以及哺乳动物微 粒体酶试验（Ames 试验），对复配剂的毒理性进行了研究。结果表明，雌小鼠LD50 为10.80 g/kg BW ，可信限为7.41~15.70 g/kg BW； 雄小鼠LD50 为12.60 g/kg BW，可信限为7.75~20.50 g/kg BW；骨髓细胞微核试验、精子畸形试验、Ames 试验的结果都为阴性，表 明香蕉果皮黄酮- 丙酸复配剂对小鼠体细胞和生殖细胞无诱变作用，也无直接或间接的致突变作用, 属实际无毒级物质。香蕉果皮黄 酮- 丙酸复配剂可作为食品防腐添加剂。 关键词：香蕉果皮;黄酮-丙酸复配剂；小鼠；毒理学 文章篇号：1673-9078(2015)7-56-60 DOI: 10.13982/j.mfst.1673-9078.2015.7.010 Toxicology of Flavonoid-propionic Acid Mixtures from Banana Peel GU Cai-qin, WEN Xiao-long, ZHU Dong-xue, LIU Peng, LAI Ya-ping, LIN Jin-ying, ZENG Qin-zhu (Department of Food , School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, China) Abstract: Crude flavonoid extract was obtained from banana peel by microwave extraction and purified using H103 macroporous resin and combined with propionic acid to produce flavonoid-propionic acid mixtures. Toxicology testing was conducted using these mixtures as per the Procedures for Toxicological Assessment of Food published by Ministry of Health of The Peoples Republic of China. Toxicology testing included using the acute toxicity test for minimum lethal dose for 50% mice (LD50), bone marrow cell micronucleus test, mice sperm morphology test, and Ames test. The results showed that the LD 50 values for female and male mice were 10 .8 and 12.6 g/kg body weight (BW), respectively; while the fiducial limits for female and male mice were 7.41~15.7 and 7.75~20.5 g/kg BW, respectively . The results fro