TILLING技术在植物功能基因组和育种中的应用.pdf

遗 传 学 报 Acta Genetica Sinica , November 2006, 33 (11)：957–964 ISSN 0379-4172 Application of TILLING in Plant Improvement 1 2 1,① WANG De-Kai , SUN Zong-Xiu , TAO Yue-Zhi 1. The Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China Abstract: TILLING (Targeting induced local lesions in genomes) is a general reverse-genetic strategy that is used to locate an allelic series of induced point mutations in genes of interest. High-throughput TILLING allows the rapid and cost-effective detec- tion of induced point mutations in populations of chemically mutagenized individuals. The technique can be applied not only to model organisms but also to economically important organisms in plants. Owing to its full of advantages such as simple procedure, high sensitivity, and high efficiency, TILLING provides a powerful approach for gene discovery, DNA polymorphism assessment, and plant improvement. Coupled with other genomic resources, TILLING and EcoTILLING can be used immediately as a haplo- typing tool in plant breeding for identifying allelic variation in genes exhibiting expression correlating with phenotypes and estab- lishing an allelic series at genetic loci for the traits of interest in germplasm or induced mutants. Key words: TILLING; reverse genetics; mutations; plant breeding Completion of the genome sequence projects of lesions, it is useful both in organisms, such as Arabi- Arabidopsis thaliana and rice (Oryza sativa L.) [1, 2] dopsis, with sophisticated gene knockout methods, has brought plant science research into a new era of