蛋白质芯片技术及电化学发光技术检测多肿瘤标志物结果的评价.pdfVIP

9．筝2 蛋白质芯片技术与电化学发光技术检测多肿瘤标志物 结果的评价 黄琛汤汉红王敏民 摘要目的：研究蛋白质芯片法与电化学发光法检测多肿瘤标志物结果的差异。方法：分别用蛋白质芯片法 和糖类抗原153(CAl53)。结果：蛋白质芯片法和电化学发光法检测12项指标的符合率均大于85％，总符合率为 90．4％。2种方法有相关性(P0．05)。结论：蛋白质芯片能正确反映肿瘤标志物的水平，且较电化学发光法简单、方 便，适合中小型医院开展。电化学发光法能更精确检测高含量肿瘤标志物。 关键词蛋白质阵列分析电化学肿瘤标记，生物学评价研究 EvaluationofMultiTumorMarkersProteinand using Chip Immunoassay HUANG Minmin Chela，TANG Hanhong，WANG DepartmentofClinicalLaboratory,Central 430014，China HospitalofWuhan，Wuhan markers Abstract evaluatethedetecteddifferenceof tumor between andelectro— Objective：1o multiple proteinchip chemiluminescence 12tumor immunoassay(ECLIA)．Methods：The 69cancer and65normalcontrolweredetected and AFP，F—PSA。PsA，CAl25，HGH，CAl53)in patients byproteinchip ECUA．Results：Thecoincidentratewas andECUAtodetect12tumormarkers．andthetwo 90．4％using proteinchip methodswere markerlevelsCanbemeasured signitleanflycorrelated伊0．05)．Conclusion：Tumor accuratelybyprotein itismore and thanECLIA．nisissuitableforthemediumandsmall-scale is chip．and simpleconveniently hospital．ECLIA moreaccuratein contentoftumormarkersthan detectinghish proteinchip． words tumor evaluationstudies protein electrochemistrymarkers，biolog