一个水稻谷胱甘肽-s-转移酶启动子的特性分析.pdfVIP

DOI 牶牨牥牣牨牭牴牫牫牤j牣cnki牣牨牥牥牬牠牫牪牰牳牣牪牥牥牱牣牥牳牣牥牨牨 河南农业科学 - S- 胡廷章 ( , 404000) - - :从水稻基因组文库中筛选得到1 个水稻谷胱甘肽 S 转移酶基因,命名为OsGS TL1 。为了研 究OsGS TL1 启动子在 物体内的表达特性,将OsGS TL1 起始位点5′- 端上游不同长度的调控序列 与报告基因GUS 融合,并在洋葱表皮瞬间表达和拟南芥中稳定表达。研究表明:在洋葱表皮细胞中, 160 bp 及更长的上游调控序列均能启动GUS 基因的表达;而在转基因拟南芥中,含有2 155 bp 的上游 序列的PGL2. 1::GUS 具有时空表达的特性,在转基因的早期幼苗中GUS 基因在子叶中特异性表达, 但在根中没有表达;而在幼苗生长的后期,根、茎、叶中都有少量的表达。但包含 1224bp 的上游序列 的PGL 1. 2::GUS 却表现为组成型表达的特性。由此推测,OsGS TL1 启动子启动的基因表达可能与 幼苗的营养代谢相关;而OsGS TL1 启动子的时空表达相关元件可能位于GST 起始位点5′- 端上游 - 2 155bp ～ - 1224bp 范围内。 - - :谷胱甘肽 S 转移酶启动子;时空表达;水稻 :S511　　:A 　　:1004 - 3268(2007)08 - 0025 - 06 Characteri ation of a Glutathione S-Transferase Promoter from Rice H U T ing-hang (Department of Biology, Cho ng qing T hree Gorg es Univer sity, Chong qing 404000, China) Abstract:OsGS TL1 gene w as isolated from the rice g enomic library . In order to investig ate the cis-elements of OsGS TL1 promoter, the prom oter regio ns with different lengths were fused to the β- glucuronidase (GUS )reporter gene. A ll constructs w ere transformed into onion epidermal cells or A . tha li ana plants to detect the expression patterns. In o nion epiderm al cells, the 160 bp frag ment and longer ones w ere functional for directing GUS ex pression. In transgenic A. tha li- ana, the 2 155 bp upstream regio n of OsGS TL1 g ene directed the GUS expre ssion only in coty le- don after germination, but not in the root of young seedlings. In the later seedling, the 2 155 bp upstream regio n of OsGS TL1 gene directed GUS expre ssion in roots, stem s, and leaves. How ev- er, the GUS gene directed by a 1224 bp upstream fragment is expressed in all the checked ti