基因多态性与β-内酰胺类抗生素过敏的关联性分析-第三军医大学学报.docVIP

IL-13及IL-4Rα基因多态性与β-内酰胺类抗生素过敏的 关联性分析 刘欣跃，董晓慧，刘青箐，王君 (兰州大学第二医院检验中心,甘肃 兰州 730030) 摘要: 目的　探讨IL-13、IL-4Rα基因多态性与中国西北地区汉族人群β-内酰胺类抗生素过敏易感性的关联性。 方法　以β-内酰胺类抗生素过敏者为研究对象，对64例β-内酰胺类抗生素过敏病例组与30例对照组进行病例对照研究，采用Sequenom MassARRAY?分子量阵列技术平台定制SNP芯片检测IL-13及IL-4Rα的4个单核苷酸多态性与甘肃地区汉族人群β-内酰胺类抗生素过敏的关联性。应用SPSS 17.0完成统计学处理，应用SHEsis软件完成IL-13的连锁不平衡分析及单倍型构建。 结果　IL-13 rs20541、IL-13 rs1881457、IL-13 rs1800925及IL-4Rα rs1801275基因多态性与β-内酰胺类抗生素过敏无显著相关性，P值0.05。进行性别分层后其结果仍无显著相关性。IL-13的CCT及CAC单倍型与中国西北地区汉族β-内酰胺类抗生素过敏的相关性有待进一步研究。 结论　IL-13及IL-4Rα基因多态性与中国西北地区汉族β-内酰胺类抗生素过敏无显著相关性。 关键词: β-内酰胺类多态性药物白细胞介素R394.6 Correlation analysis of genetic polymorphisms of IL-13 and IL-4Rα with β-lactam allergy Liu Xinyue, Dong Xiaohui, Liu Qingjing, Wang Jun (Clinical Laboratory, Second Hospital of Lanzhou University, Lanzhou, Gansu Province, China 730030) Abstract Objecttive Selecting some cytokines and their receptors such as IL-13 and IL-4Rα. Identify the relationship between these genetic polymorphisms and β-lactam allergy in Chinese Han population in northwest China. Methods We randomly selected two groups of patients with and without drug allergy against β-lactam with a case-control method. The number of case and control were 64 and 30.Investigate the correlation between four SNPs of IL-13 and IL-4Rα and β-lactam allergy by using a newly developed genotyping assay termed iPLEX? with the Sequenom MassARRAY? platform. The statistical data was treated by the SPSS 17.0 software. Haplotypes were reconstructed using the web of SHEsis. Results The results showed that the SNPs of IL-13 and IL-4Rα sush as rs20541, rs1881457, rs1800925 and rs1801275 had no significant correlation with β-lactam allergy in Chinese Han population in northwest China (p0.05). Nor gender stratification was found. The Correlation between CCT and CAC haplotypes of IL-13 and β-lactam allergy in Chinese Han population in northwest China need future research. Conclusion There was no significant correlation between IL-13 and IL-4Rα genetic polymorphisms an