列当生防菌层出镰刀菌Br_1发酵条件的优化及田间防治效果.pdfVIP

33(5)692-698 中国生物防治学报 Chinese Journal of Biological Control 2017 年 10 月 列当生防菌层出镰刀菌Br−1发酵条件的优化及田间防治效果 王亚娇，纪莉景，栗秋生，肖 颖，王连生，孔令晓* （河北省农林科学院植物保护研究所/河北省有害生物综合防治工程技术研究中心/农业部华北北部作物有害生物综合治理重点实验室，保定 071000 ） 摘要：列当是根寄生杂草，很难防除。菌株 Br−1 是从感病列当中分离出的对列当有较高防治效果的层出 镰刀菌。为了提高菌株 Br−1 的产孢量，本研究通过单因素试验和正交设计试验对其培养基和发酵条件进 行了优化。结果表明，菌株 Br−1 适宜的发酵培养基为葡萄糖 1%，NH NO 0.2% ，KH PO 0.3% ，MgSO ·7H O 4 3 2 4 4 2 0.1%；优化后的培养条件为：最初pH 5.0 ，培养温度24 ℃，装液量20% ，振荡速度 190 r/min，发酵时间 6 d。较优化前的初始培养基和培养条件产孢量提高了 257.5 倍。在田间防治试验中菌株 Br−1 对弯管列当 的防治效果最高可达 67.2%。 关 键 词：层出镰刀菌；发酵优化；培养基；防治效果 中图分类号：S476 文献标识码：A 文章编号：1005-9261 (2017)05-0692-07 Optimization of Fermentation Medium Components and Conditions of Fusarium proliferatum Br-1 and Its Field Control Efficacy * WANG Yajiao, JI Lijing, LI Qiusheng, XIAO Ying, WANG Liansheng, KONG Lingxiao (Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture/IPM Center of Hebei Province/Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, China) Abstract: Biological control agent Br-1 was isolated from diseased broomrape and identified as Fusarium proliferatum . In order to increase the sporulation of strain Br-1, the medium components and fermentation conditions were optimized by single factor and orthogonal design. The results indicated that optimized medium components of strain Br-1 were glucose 1%, NH NO 0.2%, KH PO 0.3%, MgSO ·7H O 0.1%. Optimum 4 3 2 4