影响泽泻蕨孢子培养的环境因素研究-热带亚热带植物学报.pdfVIP

热带亚热带植物学报　2015 ，23(4) ：4 13 ~ 4 18 Journal of Tropical and Subtropical Botany 影响泽泻蕨孢子培养的环境因素研究 1 2 3* 1,3 1 莫日根高娃, 谭桂娟, 严岳鸿 , 赵国华 , 刘保东 (1. 哈尔滨师范大学生命科学与技术学院，哈尔滨 150025； 2. 北京吉鼎立达生物科技有限公司，北京 101105； 3. 中国科学院上海辰山植物科学 研究中心，上海辰山植物园，上海 201602) 摘要： 为了解环境 因子对泽泻蕨(Hemionitis arifolia Moore.)培养 的影响 ，采用孢子培养技术研究温度 、光照、pH 、密度等对其有 性繁殖 的影响。结果表 明，泽泻蕨孢子为需光萌发 ，孢子萌发和配子体发育 的最适环境条件为 ：温度 25℃，日光灯光照强度为 150 µmol m–2s– 1 ，pH 为 6.5 ，播种密度为 5~8 grains cm–2 ，幼苗管护简单 ，成活率较高 ，未见病虫害。这为保护和开发我 国泽泻蕨 资源提供 了理论依据和技术支持 。 关键词： 珍稀植物 ；泽泻蕨 ；孢子培养 ；环境 因素 doi: 10.11926/j .issn.1005–3395.2015.04.008 Effects of Environment Factors on Spores Culture of Hemionitis arifolia Moore. 1 2 3* 1,3 1 Morigengaowa , TAN Gui-juan , YAN Yue-hong , ZHAO Guo-hua , LIU Bao-dong (1. College of Life Sciences and Technology, Harbin Normal University, Harbin 150025, China; 2. Beijing Jidinglida Biological Technology Co., LTD, Beijing 101105, China; 3. Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Chenshan Botanical Garden, Shanghai 201602, China) Abstract: In order to understand the influence of environment factors on culture of Hemionitis arifolia Moore., the effects of temperature, light condition, pH and density on sexual reproduction of spores were studied by micro culture. The results showed that the germination of H. arifolia spores need light, the optimum condition for spore germination and gametophyte development of H. arifolia was under 25 ℃, 150 µmol m–2 s–1 of light intensity, pH =6.5 with sowing density of 5–8 grains cm–2 . The management of seedlings was simple. The survival of H. arifolia was high with few disease and insect pest. So, these would provide the technical assistance and theoretical foundation for protection and development of H. arifolia resources. Key words: Rare plant; Hemioniti