丛枝菌根真菌对黄瓜枯萎病的影响.pdfVIP

Mycosystema 菌 物 学 报 15 May 2008, 27(3): 395-404 jwxt@ ISSN1672-6472 CN11-5180Q ©2008 Institute of Microbiology, CAS, all rights reserved. 丛枝菌根真菌对黄瓜枯萎病的影响 1* 2 王倡宪 郝志鹏 1 黑龙江大学农业资源与环境学院 哈尔滨 150080 2 中国农业大学资源与环境学院 北京 100094 摘 要：盆栽条件下播种黄瓜 Cucumis sativus 同时接种丛枝菌根真菌 Glomus etunicatum，4 周后对接种处 理和对照黄瓜苗分别浇灌 Fusairum oxysporum f. sp. cucumerinum 分生孢子悬液，2 周后测定幼苗生物量、 根内丙二醛、可溶性糖与游离脯氨酸含量及根围真菌和细菌数量。结果表明：接种 Glomus etunicatum 根 系干重增加了 9.3%，提高了根内可溶性糖和游离脯氨酸含量，显著减少了根围真菌数量，降低了黄瓜枯 萎病的发病率和病情指数。而不接种 Glomus etunicatum 的黄瓜苗根系干重减少了 28.0% 。研究认为AM 真菌 Glomus etunicatum 对黄瓜枯萎病具有一定的生防价值。 关键词：生物量，可溶性糖，游离脯氨酸，根际细菌，根际真菌 Effects of arbuscular mycorrhizal fungi on fusarium wilt of cucumber seedlings 1∗ 2 WANG Chang-Xian HAO Zhi-Peng 1College of Agricultural Resources and Environmental Sciences, Heilongjiang University, Harbin 150080, China 2 College of Natural Resources and Environmental Sciences, China Agricultural University, Beijing 100094, China Abstract: Pot experiment was conducted to explore whether nursery inoculation of cucumber with Glomus etunicatum could alleviate fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum. Four-week-old seedlings inoculated with Glomus etunicatum were infected with F . oxysporum f. sp. cucumerinum by pouring 基金项目：黑龙江省教育厅科学技术研究面上项目（No.）；黑龙江大学校青年基金（No. QL200530 ）；黑龙江大学博 士启动金（No. 200504） * Corresponding author. E-mail: changxianwang@ 收稿日期：2007-09-18 ，接受日期：2008-02-25 396 Mycosystema conidial suspension. Biomass, contents of malonaldehyde (MDA), soluble sugar and free proline in roots, as well as the quantity of bacteria and fungi in rhizosphere were determined. The results indicated that the root