海藻糖合成酶基因的克隆及序列分析.docVIP

Clone and Sequence Analysis of Trehalose Synthesis from Pseudomonas Stutzeri Yan Yu Qing, Zhang Li Juan, Li Xin Ling, Xu Xiang Ling* Biology Department of Harbin Normal University, Harbin, Heilongjiang 150080, China; yanyuqing88@ Abstract: We have cloned Trehalose Synthesis gene using PCR from Pseudomonas Stutzeri. We linked this gene with pGEM-T-Easy Vector , analysis the whole gene sequence and transformation the recombinant gene into Escherichia coli JM109 .The result of sequence of this gene showed :cloned gene whole length is 2070 bp, has 96.66% homology compared with recorded sequence AF113617in GenBank, encoded 689 amino acids , has 99.71% homology compared with AF113617 . This cloned gene has been recorded by GenBank, accession number is DQ452614. [Nature and Science. 2006;4(4):26-31]. Keywords: Trehalose Synthesis; TreS gene; PCR clone; sequence analysis Introduction Trehalose is a deoxidizing disaccharide containing two glucoses which are combined with α，α glucosidic linkage. It is abundant in animals, plants and microbe. Trehalose has an important function in protecting plant stress tolerance. It usually produced in the condition of intimidating. The content of the trehalose varys as the condition changes. Trehalose is a irritability metabolite. The reason that why some species performance stress tolerance in inclemency environment is trehalose can have the protection effect on biological giant molecule, such as biomembrane, protein and nucleic acid etc. So according to that, the species which are rich in trehalose can show the peculiar biological speciality. The function of the biological protection of trehalose is that it strongly binds up the water molecules, owning the bound water together with membrane lipid. Or it can instead the function of combining water with the membrane. Consequently preventing the denaturalization of biomembrane and membrane protein. Because of the potential applications of the trehalose on food, cosmetic, medicame