环介导等温扩增技术在肠杆菌科致病菌检测中的研究进展.pdfVIP

312 2014, Vol.35, No.19 食品科学 ※专题论述 环介导等温扩增技术在肠杆菌科致病菌 检测中的研究进展 1 2 3 2 4,* 何晓华 ，顿玉慧，卢 力，李 可，刘 斌 （1.西北农林科技大学植物保护学院，陕西 杨凌 7 12100 ；2.浙江省检验检疫科学技术研究院，浙江 杭州 3100 16 ； 3.义乌市检验检疫科学技术研究院，浙江 义乌 322000 ；4.西北农林科技大学食品科学与工程学院，陕西 杨凌 7 12100 ） 摘  要：环介导等温扩增（loop-mediated isothermal amplification，LAMP ）技术是近年来新发展起来的一种恒温核酸 扩增技术，该技术具有设备简单、检测速度快、灵敏度高、特异性强以及扩增效率高等优点，已被广泛地应用于食源 性致病菌的检测研究。本文重点阐述近年来国内外学者应用LAMP技术在肠杆菌科中常见致病菌的检测研究进展。 关键词：环介导等温扩增；肠杆菌科；致病菌；检测 Recent Progress in Application of Loop-Mediated Isothermal Amplification Method for Detection of Enterobacteriaceae Pathogens 1 2 3 2 4,* HE Xiao-hua , DUN Yu-hui , LU Li , LI Ke , LIU Bin (1. College of Plant Protection, Northwest AF University, Yangling 7 12100, China; 2. Zhej iang Academy of Science Technology for Inspection Quarantine, Hangzhou 3100 16, China; 3. Yiwu Academy of Science Technology for Inspection Quarantine, Yiwu 322000, China; 4. College of Food Science and Engineering, Northwest AF University, Yangling 7 12100, China) Abstract: In recent years, loop-mediated isothermal amplification (LAMP) has emerged as a new technology for nucleic acid amplification under constant temperature conditions, which is characterized by simple instrumental requirements, rapid detection, high sensitivity, high specificity, and efficient amplification. This technology has been widely applied in the detection of foodborne pathogens. This paper focuses on the recent progress in the application of LAMP assay in the detection of common Enterobacteriaceae pathogens. Key words: loop-mediated