营养胁迫条件与耐药乳腺癌细胞生长优势的相关性研究.pdfVIP

中国细胞生物学学报 Chinese Journal of Cell Biology 2017, 39(9): 1173– 1177 DOI: 10.11844/cj cb.2017.09.0047 营养胁迫条件与耐药乳腺癌细胞 生长优势的相关性研究 蔡燕飞 马 鑫 张 鹏 陈 蕴 金 坚* (江南大学药学院, 药物设计与分子药理学实验室, 无锡2 14 122) 摘要 与正常组织相比, 实体瘤生长的微环境更加恶劣。那么, 为何在如此恶劣的生长环境 下, 肿瘤细胞仍能顽强生存, 更有胜者转化成耐药肿瘤细胞, 造成临床难以治疗的困难局面。该文 拟以低营养模拟肿瘤的内部环境, 研究野生型与耐药型细胞生长状态和生长需求存在的差异, 进而 解析其具体原因。该文以人乳腺癌MCF-7细胞的野生型和耐阿霉素型细胞为研究对象, 通过对细 胞增殖能力、细胞迁移和血管内皮生长因子(vascular endothelial growth factor, VEGF)分泌等实验 分析发现, 在低营养条件下, 耐药型细胞的增殖能力和迁移能力远高于野生型细胞, 而且耐药型细 胞能分泌更多的VEGF 。该研究结果表明, 在营养胁迫条件下, 耐药型细胞的生存能力更强, VEGF 的高分泌可能是促进其快速生长的重要原因。 关键词 营养胁迫; 耐药; 肿瘤; VEGF The Study in Correlation between Nutrient Stress and Growth Advantage of Drug Resistance Cancer Cells Cai Yanfei, Ma Xin, Zhang Peng, Chen Yun, Jin Jian* (The Laboratory of Drug Design and Molecular Pharmacology, School of Pharmaceutics, Jiangnan University, Wuxi 214122, China) Abstract The microenvironment of solid tumor is worse than the normal tissue. But why the tumor cells can still survive and transform into drug resistant tumor cells which causing difficulties on clinical treatment is not clear. This study proposed low nutrition as simulation environment in solid tumor, and investigated the growth status and growth demand of wild type and drug resistant cells, then parsed the specific reason. The wild type and drug resistant breast cancer MCF-7 cells were used to detect cell proliferation, cell migration and the expression level of vascular endothelial growth factor (VEGF). It is found that under the low nutrition condition, the proliferation and migration abilities of drug resistant cells are stronger than that of the wild type cells. What’s more, the VEGF also secrete more in drug resistant cells. These results