黄瓜枯萎病菌遗传多样性的aflp分析-植物病理学报.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA摇 41(3):301鄄304(2011) 藘蕛蕛蕛蕛蕛藘蕛 蕛研究简报藘 藘蕛蕛蕛蕛蕛 黄瓜枯萎病菌遗传多样性的AFLP分析 * 张艳菊 , 陈 霞, 刘 东, 秦智伟, 周秀艳, 蒲子婧 (东北农业大学, 哈尔滨 150030) AFLP analysis of genetic diversity of Fusarium oxysporum f. sp. cucumainum摇 ZHANG Yan鄄Ju, CHEN Xia, LIU Dong, QIN Zhi鄄Wei, ZHOU Xiu鄄Yan, PU Zi鄄jing摇 (Northeast Agricul鄄 tural University,Harbin 150030,China) Abstract: The genetic diversity of Fusarium specises isolated from cucumber plants showing wilt disease from8 citieswas detectedby amplifiedrestriction fragment polymorphism (AFLP) . Sixteen combinationsof primer pair were selected from 130 combinations and used to amplify the genomic DNA of 351 Fusarium oxysporum isolates. A total of 116 444 bands were obtained and 60 579 of them showed polymorphism, accounting for 52.0%. The genetic distances of351F. oxysporum isolates were ranged0.56鄄1.00 based on AFLP markers,averaged at 0.78. The results showed that the genetic diversity among the F. oxysporum isolateswashigh. The 351 tested isolates could be classified into 5 AFLP groups,and most of the isolates from the same area were clustered in the same group by using the UPGMA method. Key words: cucumber;Fusarium oxysporum;AFLP;genetic diversity 文章编号:0412鄄0914(2011)03鄄0301鄄04 [4] 摇 摇 黄瓜枯萎病是由半知菌亚门尖孢镰刀菌黄瓜 信息量小以及 RAPD 技术不稳定的缺点 。 该技 专化型(Fusarium oxysporum f. sp. cucumainum 术以其重复性好、效率高的特点已被广泛应用于动 Owen)侵染引起的一种土传病害,是影响黄瓜生产 物、植物研究,也成功地应用于植物病原真菌研究 [1] 中,但在黄瓜枯萎病菌的研究中报道较少。 因此, 的最主要病害之一 。 近年来随着分子生物学技 术的迅速发展,国内外学者对于病原真菌的遗传多 本研究采用 AFLP 分子标记方法对从哈尔滨、长 [2] 春、沈阳、北京、保定、银川、西宁及杭州8个地区发 样性做了大量的研究,Wang等 对影响黄瓜枯萎 病菌AFLP技术体系的多种因素作了探讨,得到了 病的黄瓜植株上分离得到的尖孢镰刀菌进行分析, 1种适合于黄瓜枯萎病菌AFLP分析的优化体系; 旨在为更好的