黑龙江核盘菌致病性分化研究-中国农学通报.pdfVIP

中国农学通报 2015,31(5): 102-107 Chinese Agricultural Science Bulletin 黑龙江省核盘菌致病性分化研究 刘春来，王 爽，夏吉星，杨 帆，李新民，孟庆林，张匀华 （黑龙江省农业科学院植物保护研究所，哈尔滨150086） 摘 要：为进一步明确核盘菌种群内致病力分化现象，采用离体叶片菌饼接种方法，以向日葵品种‘丰葵 杂1号’为供试寄主，对采集自黑龙江省6 个地区18 个县的大豆和向日葵两种寄主植物的93 个核盘菌菌 株进行了致病性测定。结果表明，除14 个菌株不能致病外，其余79 个菌株均能引起向日葵叶片发病，但 所致病斑直径的大小差异很大(0.2~5.3 cm) ，中等致病力菌株数量较多，共占总菌株数的66.7%。来源于 同一地区以及同一地块的菌株其致病性也达到了极显著差异，说明在所测定的核盘菌分离株内确实存 在着致病力强弱明显不同的菌株，这种致病力强弱明显不同的现象与菌株来源地区和寄主来源间均无 明显的关系。 关键词：核盘菌；致病性分化；向日葵；离体叶片接种法 中图分类号：S435.65 文献标志码：A 论文编号：2014-2465 StudyonPathogenicityDifferentiationofSclerotiniasclerotiorumIsolatedfrom DifferentRegionsinHeilongjiangProvince LiuChunlai,WangShuang,XiaJixing,YangFan,LiXinmin,MengQinglin,ZhangYunhua (PlantProtectionInstituteofHeilongjiangAcademyofAgriculturalSciences,Harbin150086) Abstract:InordertoconfirmpathogenicitydifferentiationwithinSclerotiniasclerotiorum groups,ninety-three isolatesof Sclerotiniasclerotiorum isolatedfromsoybeanandsunflowerindifferentregionsofHeilongjiang Provinceweretestedfortheirpathogenicityto ‘FengkuizaNO1’varietybythemethodofplacingthemycelia plugsontheexcisedleaves.Theresultsofinoculationonexcisedleavesof ‘FengkuizaNO1’showedthat,79 isolateshadpathogenicityalthoughthelesionsizesrangedfrom0.2cmto5.3cmindifferentisolates.However, nopathogenicitywasfoundinfourteenisolates.Thenumberofmoderatepathogenicityisolatesof Sclerotinia sclerotiorum were more than others and 66.7% proportion in the pathogen population. Furthermore, the extremelysignificantdifferencesofpathogenicitywerefoundamongisolatesevenderivedfromthesameregion andthesamefield.Theresultsindicatedthat,thephenomenonofpathogenicitydifferentiationwasexisted amongisolates,whichwasnosignificantrelationshiptogeographicalloc