角膜内皮细胞再生研究新进展-中华细胞与干细胞杂志.pdfVIP

( ) 2014 5 4 2 Chin J Cell Stem Cell (Electronic Edition), May 2014,Vol. 4, No. 2 116 中华细胞与干细胞杂志电子版 年 月第 卷第 期 •综述• 角膜内皮细胞再生研究新进展 朱梦玉 邵春益 傅瑶 【摘要】人角膜内皮细胞的主要功能是维持角膜透明性，角膜内皮单层发育成熟形成 细胞接触后，内皮细胞会停止分裂增殖，但并没有退出细胞周期。角膜内皮细胞的增殖有 G1 TGF-β2 多种因素的参与和影响，接触抑制和 期抑制使细胞增殖暂时停止；细胞因子 抑 S EGF FGF NGF ROCK 制人角膜内皮细胞进入细胞周期 期，而 、 、 则能够促进细胞的增殖； 抑制剂Y-27632 能够促进角膜内皮细胞的粘连，有助于内皮细胞的损伤修复。体外培养角 膜内皮前体细胞、诱导多潜能干细胞向角膜内皮细胞分化，为今后治疗角膜内皮失代偿提 供了新方向。 【关键词】角膜； 内皮细胞； 细胞周期； 细胞增殖； 干细胞 Research progress on corneal endothelial cells regeneration Zhu Mengyu, Shao Chunyi, Fu Yao. Department of Ophthalmology, Ninth people’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China Corresponding author: Fu Yao, Email: fuyaofy@ Abstract Human corneal endothelial cells play a key role in maintaining corneal 【 】 transparency. Division of the cells cease and arrest in G1 phase when cell-cell contact formed, but they don’t exit the cell cycle. There are many factors involved in corneal endothelial cell proliferation, TGF- β2 inhibits human corneal endothelial cells into the S phase of the cell cycle, whereas EGF, FGF, NGF can promote cell proliferation; ROCK inhibitor Y-27632 can promote adhesion of endothelial cells. Culture of corneal endothelial precursor cells, and inducing pluripotent stem cells into corneal endothelial cell may provide a new method for the treatment of corneal endothelial dysfunction. Key words Cornea Endothelial cells Cell cycle Cell proliferation Stem 【 】 ；  ；  ；  ；  cell 5