He-Ne激光处理与干旱胁迫对小麦幼苗保护酶活性及脂-植物生态学报.PDF

植物生态学报 2008, 32 (5) 1002~1006 Journal of Plant Ecology (Chinese Version) He-Ne激光处理与干旱胁迫对小麦幼苗保护酶活 性及脂质过氧化作用的影响 1, 2 1 1 1 1* 邱宗波 刘 晓 李方民 田向军 岳 明 (1 西部资源生物与现代生物技术省部共建教育部重点实验室(西北大学),西安 710069) (2 河南师范大学生命科学学院,河南新乡 453007) 摘 要 用He-Ne激光(5.23 mW ·mm–2)处理经5%、10%、15% PEG6000胁迫的小麦幼苗, 分析了干旱胁迫条件下 激光处理对小麦幼苗保护酶活性及脂质过氧化作用的影响。适度干旱胁迫的小麦幼苗经He-Ne激光辐照后, 丙二 – 醛(MDA)含量和超氧自由基(O2 .)产生速率显著降低(p 0.05), 而过氧化物酶(POD)活性和抗坏血酸(AsA)、谷胱甘 肽(GSH)含量却显著增加(p 0.05) 。总体上看, 5%和10% PEG6000胁迫的小麦幼苗经激光辐照3 min后抗旱性增强。 关键词 He-Ne激光 小麦 干旱胁迫 保护酶 脂质过氧化 INFLUENCE OF He-Ne LASER IRRADIATION ON PROTECTIVE ENZYME ACTIVITIES AND LIPID PEROXIDATION IN WHEAT SEEDLINGS BY DROUGHT STRESS DAMAGE 1,2 1 1 1 1* QIU Zong-Bo , LIU Xiao , LI Fang-Min , TIAN Xiang-Jun , and YUE Ming 1Key Laboratory of Resources Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi’an 710069, China, and 2 College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China Abstract Aims We studied protective enzyme activities and lipid peroxidation in wheat seedlings treated with different concentration of PEG6000 to determine the effects of He-Ne laser on wheat (Triticum aestivum) exposed to drought stress. Method We treated 12-day-old, drought-stressed seedlings with 5%, 10% and 15% PEG6000 for 3, 6, 9, 12 and 15 days. After 3, 6, 9, 12 and 15 days of drought stress, a He-Ne laser was employed to irradi- ate seedlings of spring wheat for 0, 1 and