昆虫血清素受体蛋白的生物信息学分析-应用昆虫学报.pdfVIP

Chinese Journal of Applied Entomology 2015, 52(3): 631640. DOI: 10.7679/j.issn.20951353.2015.074 昆虫血清素受体蛋白的生物信息学分析* 常菊花1** 何月平2*** 1. 434025 2. 430070 摘 要 【目的】5-5 5- 【方法】 7 5-23 NCBI 5-【结果】47 5-40 5-7 5-7 G 5-【结论】5- 5- 5-HT 5- 关键词 5- Bioinformatic analysis of insect serotonin receptor proteins 1** 2*** CHANG Ju-Hua HE Yue-Ping (1. College of Life Science, Yangtze University, Jingzhou 434025, China; 2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China) Abstract [Objectives] There are five subtypes of serotonin receptor proteins of insects. The relationship between protein structure and the evolution of serotonin receptors in insects was comprehensively studied. [Methods] First, the bioinformatics of 23 protein sequences of serotonin receptors in seven insects reported in the literature were analyzed; Second, the protein sequences of serotonin receptors in the NCBI database were verified by multiple sequence alignment and constructing a phylogenetic tree. [Results] The results show that 40 of 47 putative protein sequences were those of insect serotonin receptors, whereas the other 7 protein sequences, which had 7 transmembrane regions, belong to the G protein-coupled receptor superfamily. It is uncertain if these 7 proteins are insect serotonin receptors. [Conclusion] This study provides a basis for analyzing the function of insect serotonin receptors. Key words insect, serotonin receptor, bioinformatics analysis, phylogenetic tree 5-5-hydroxytryp tamine5-HT 5-