丹蛭降糖胶囊对糖尿病大鼠肾组织nf-κb表达的影响-newcentury.pdfVIP

Global Journal of Endocrinology and Metabolism Studies，2014,Vol.1,No.1 /index.php/gjems The influence of Danzhi Jiangtang Capsule on the expression of NF- κB in kidney tissue of diabetic rats XIONG Yuan-yuan, LI Zhong-nan, LIU Shan-shan, ZHANG Fan, ZHANG Pei-pei Department of Endocrinology, the First Affiliated Hospital, Anhui University of Traditional Chinese Medicine, Hefei, Anhui, China Received: Sep 26, 2014 Accepted: Sep 29, 2014 Published: Oct 10, 2014 DOI:10.14725/gjems.v1n1.a827 URL:/10.14725/gjems.v1n1.a827 This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Objective: To investigate the expression level of NF- κ B in renal tissue of diabetic rodent model and the regulating action of Danzhi Jiangtang Capsule (DJC) . Methods: 55 male SD rats were randomly divided into 2 groups, normal control group and experimental group. Normal control group was fed with basal diet, the experimental group were fed with high calorie diet. A single intraperitoneal injection of streptozotocin (STZ) of 35mg/kg to establish the animal model of diabetes mellitus, blood glucose was measured after 120h,when the diabetic rodent model was built, the experimental group were randomly divided into 4 groups, high does group of DJC, low does group of DJC , pioglitazone group and model group. In addition to the model group and control group, each group was kept with continuous intragastric administration of drugs for 8 weeks, high does group of DJC, low does group of DJC were treated with 1.08g/ (kg • d), 0.54 g/ (kg • d) by gavage, pioglitazone group was treated with 10mg/ (kg • d) by gavage, the blank group and the model group were treated with physiological saline 5ml/ (kg • d) by gavage. Then determined NF-κB by immunohistochemical method and detected bloo