根毛生长过程中ROP的分布.PPT

(c) A BLC cryofixed by high-pressure freezing and subsequent freeze substitution. BLC are characterized by the presence of numerous mitochondria, Golgi stacks and secretory vesicles. Scale bar = 1 mm. Part (c) Abbreviations: CW, cell wall; G, Golgi stack; M, mitochondria; SV,secretory vesicles. (d) Unlike Arabidopsis BLC, border cells of the root tip of cotton disperse into suspension within seconds when root tips are dipped in water. Cotton root tip releases 10 000 border cells per day. Scale bar = 1 mm. Part (d) Figure 1. Organization of Arabidopsis border-like cells (BLC) and cotton border cells. (a) Micrograph of a root tip showing BLC detaching from the cap in layers of cells. Scale bar = 100 mm. (b) An isolated block of Arabidopsis BLC stained with Calcofluor. Note that BLC still remain associated in layers and do not disperse into solution. Scale bar = 50 mm. Immunofluorescence staining of Arabidopsis thaliana BLC with anti-pectin and anti-AGP antibodies. (a) A young root tip showing one layer of detaching BLC. Scale bar = 50 mm. (b) BLC are strongly labelled with JIM5 specific for pectin homogalacturonan. Scale bar = 50 mm. (c) BLC are also strongly labelled with the mAb JIM13 specific for AGPs. Scale bar = 100 mm. The pattern of BLC organization appears to be specific to the Brassicaceae family. (a) BLC of Brassica napus （欧洲油菜）still attached to the root apex(Ruthenium Red staining). Scale bar = 50 mm. (b) BLC of Brassica oleraceae （青花菜）stillattached to the root apex (Calcofluor staining). Scale bar = 100 mm. (c) BLC of Sinapis alba that have detached from the root apex (Ruthenium Red staining).Scale bar = 50 mm. Morphological phenotypes of root tips showing border-like cells (BLC) of the wild type and cell wall mutants of Arabidopsis. Border-like cells are released from the root tip in organized cell layers (arrows) in the wild type and in all mutants examined with the exception of qua1-1 and qua2-1. Note also that border-like cell organization is simi