激光熔覆技术制备的纳米钽-钛合金棒结构特征及-南方医科大学学报.PDF

基础研究 激光熔覆技术制备的纳米钽激光熔覆技术制备的纳米钽--钛合金棒结构特征及生物相容性钛合金棒结构特征及生物相容性 1 1 1 2 1 邹 琳 ，江建明 ，杨 勇 ，陈长军 ，瞿东滨 1 2 南方医科大学南方医院脊柱骨科，广东 广州 510515 ；苏州大学，江苏 苏州 215006 摘要：目的运用激光熔覆技术制备新型纳米钽-钛合金棒并研究其结构特征及生物相容性，为临床选用内固定提供依据。方法 以纳米级钽粉（Ta ，Tantalum）为原料，利用激光熔覆技术制备两侧具有对称纳米钽涂层的新型钛合金棒。SEM 观察微观界面 结构、测量孔隙直径并分析金相成分。采用MTT 细胞毒性试验和ALP 活性检测评价新型纳米钽-钛合金对MC3T3-E1细胞增 殖和分化性能的影响。结果 扫描电镜下观察到试样的Ta 涂层表面呈粗糙多孔结构，钽粉颗粒间致密熔融，孔隙直径平均在 200~300 μm 之间，加工过程前后成分一致；成分分析结果显示熔覆过程并未产生新的元素。两组材料皆可明显促进MC3T3-E1 细胞增值，但新型纳米钽-钛合金性能更为优异，同时能够促进成骨细胞的骨化，差异具有统计学意义。结论 激光熔覆技术可 成功实现钽与医用钛合金的冶金结合，并使其拥有良好的表面结构和生物相容性。 关键词：纳米钽；多孔；激光熔覆；微区组织结构；生物相容性 Study on Structure characteristics and biocompatibility of new-model Nano-Ta-Ti alloy rod fabricated by laser melting technique 1 1 1 2 1 ZOU Lin , JIANG Jianming , YANG Yong , CHEN Changjun , QU Dongbin 1Department of Spinal Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; 2 SooChow University, Suzhou 215006, China Abstract: Objective To evaluate the structural characteristics and biocompatibility of a novel nano-Ta-Ti alloy rod for its potential application in internal fixation. Methods Ta coating of a Ti alloy rod with nano-Ta (tantalum) powder was performed using laser melting with symmetrical grooves repleted with nano-Ta powder along the whole length. The microstructure of the cross section of Ta-Ti alloy rod, pore diameter and components of the coating were analyzed using scanning electron microscopy. The influence of this nano-Ta-Ti alloy on proliferation and differentiation of MC3T3-E1 cells was evaluated by MTT cytotoxicity test and ALP activity test. Results Under scanning electron microscope, the Ta-coating surface presented with a gross porous (