离子色谱法测定果汁中乳酸富马酸柠檬酸的含量-ThermoFisher.PDFVIP

23 3 Vol. 23No. 3 57 2014 5 CHEMICAL ANALYSIS AND METERAGE May 2014 doi 10.3969j.issn2014.03.016 1 2 1 3 [1. 710068 2. 710003 3. ( ) 100080] Onguard II RP IonPac AS19(250 mm4 mm) IonPac AG19(250 mm4 mm) 0.999 90.7%102.8% n 0.41%1.43%( =6) mgL O657.7 A 1008 6145(2014)03 0057 04 Determination of Lactate, Fumarate and Citrate in Fruit Juice by Ion Chromatography 1 2 1 3 Wang Changzhao Guo jie Wu Shuangmin Li Renyong [1. Shaanxi Entry Exit Inspection and Quarantine Bureau Xian710068 China 2. Xian Grain and Oil Quality Inspection Center Xian710003 China 3. Thermo Fisher Scientific(China) Co., Ltd. Beijing100080 China] AbstractA new method for determination of lactatefumarate and citrate in fruit juice was developed and validated based on ion chromatography. Onguard II RP cartridges were used to remove hydrophobic substances in the fruit juice. The content of lactatefumarate and citrate in fruit juice were determined by using IonPac AS19 chromatographic column and IonPac AG19 guard column, potassium hydroxide gradient elution and circulation suppression conductometric. The calibration curves of correlation coefficients were more than 0.999 for these organic acids. The recoveries were between n 90.7% 102.8% and the relative standard deviation of determination results was between 0.41% 1.43%( =6). The detection limits were mgL for lactatefumarate and citrate, respectively.