蒸汽爆破玉米秸秆酶解动力学-微生物学报.PDFVIP

生 物 工 程 学 报 Chin J Biotech 2011, April 25; 27(4): 592−597 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn ©2011 CJB, All rights reserved. 工业生物技术 蒸汽爆破玉米秸秆酶解动力学 1,2 2 1 2 任省涛 ，程可可 ，宋安东 ，张建安 1 河南农业大学生命科学学院，郑州 450002 2 清华大学核能与新能源技术研究院，北京 100084 摘 要: 为了掌握蒸汽爆破玉米秸秆的酶解特性，研究了不同底物浓度、酶浓度、温度对反应速率的影响。运用米氏 方程对酶解动力学过程进行拟合，结果表明，纤维素酶对该玉米秸秆的水解反应在反应前3 h 符合一级反应，可用米氏 方程对其进行拟合。在转速为 120 r/min、酶浓度为 1.2 FPU/mL、pH 5.0 、温度为45 ℃时米氏常数Km 为 11.71 g/L， 最大反应速率 Vm 为 1.5 g/(L·h)。确立了包括底物浓度、酶浓度、温度在内的酶解动力学模型，该模型适合温度为 30 ℃~50 ℃。 关键词: 米氏方程，玉米秸秆，酶解，动力学，纤维素酶 Kinetics of enzymatic hydrolysis of steam-explosion pretreated corn straw 1,2 2 1 2 Shengtao Ren , Keke Cheng , Andong Song , and Jian’an Zhang 1 College of Life Science, Henan Agricultural University, Zhengzhou 450002, China 2 Institute of Nuclear and New Energy Technology , Tsinghua University, Beijing 100084, China Abstract: In order to learn the enzymatic hydrolysis characteristics of steam-explosion pretreated corn straw by cellulase, the effects of substrate concentration, cellulase concentration and temperature were determined. The kinetics of the hydrolysis reaction could be described with the Michealis-Menten equation, and the hydrolysis reaction obeyed the classical first-order reaction rate in the first three hours. In the condition of 45 °C and pH 5.0 and the stirring rate 120 r/min, the Michealis constant (Km) and maximum rate ( V ) for 1.2 FPU/mL of cellulase were 11.71 g/L and 1.5 g/(L·h). The kinetic model, including the parameters such as m s